Caspase-14 Antibody (YA3180)
(Synonyms: CASP14; Caspase-14; Caspase 14; MICE; CASP-14)
Based on 1 Customer Validation
Caspase-14 Antibody (YA3180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-14.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:100-1:200 | 1:50-1:200 | 1:50-1:100 | 1:50 |
Product Details
Caspase-14 Antibody (YA3180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-14.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 28 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 28 kDa
Entrez Gene: 23581 Human ; 12365 Mouse ; 299587 Rat
SwissProt: P31944 Human ; O89094 Mouse ;
OMIM: 617320 Human
A synthesized peptide derived from human Caspase-14 aa101-155.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Caspase-14 is a Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF. Involved in processing of prosaposin in the epidermis. May be involved in retinal pigment epithelium cell barrier function. Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD[1][2][3][4][5].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:Expressed in keratinocytes above the basal layer of adult skin (from the spinous layer to the granular layer and stratum corneum) (protein level) . Expressed in keratinocytes of hair shafts and sebaceous glands (protein level) . Expressed at very low levels in psoriatic skin (PubMed:11175259) . The mature form p17/10 is expressed in the stratum corneum of the epidermis, and the intermediate form p20/p8 is expressed in the granular cells of the upper granular layer of the epidermis (PubMed:22825846) .
Induction:In undifferentiated keratinocytes under postconfluency growth conditions (in vitro) (PubMed:11175259) . By high glucose in retinal pigment epithelia cells (PubMed:25121097) -
Subunit
Heterodimer of a large and a small subunit, both processed from the precursor; the mature active form is a p17/p10 dimer and the intermediate form a p20/p8 dimer
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SwissProt ID
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Synonyms
CASP14; Caspase-14; Caspase 14; MICE; CASP-14
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Research Field
Cell Biology
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