Copy Link Print Information

ROCK2 Antibody (YA1667)

(Synonyms: p164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha)
Customer Review

Based on 1 Customer Validation

ROCK2 Antibody (YA1667) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ROCK2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • ROCK2 Antibody (YA1667) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

ROCK2 Antibody (YA1667) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ROCK2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 161 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 161 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ROCK2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104018

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ROCK2 Antibody (YA1667)
    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg), using ROCK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
    Immunocytochemistry analysis of Hela cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
    Immunocytochemistry analysis of A431 cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ROCK2 (Rho-associated coiled-coil-containing protein kinase 2) is a serine/threonine kinase that functions as a major downstream effector of Rho GTPases and regulates cytokinesis, smooth muscle contraction, actin stress fiber formation, focal adhesion dynamics, and transcriptional responses linked to cytoskeletal remodeling[1][2]. Mechanistically, ROCK2 promotes actomyosin contractility through signaling networks that control actin organization, cell morphology, migration, proliferation, adhesion, and inflammatory responses, placing it at the center of cytoskeleton-dependent cellular regulation[3][5]. Because these processes govern tissue homeostasis and cellular adaptation, dysregulated ROCK signaling has been implicated in cardiovascular, neurodegenerative, inflammatory, and cancer-associated pathologies[3][2]. In experimental disease models, ROCK2 has been identified as an important regulator of neuronal survival, axonal stability after injury, and regenerative responses, supporting its utility for studying neurodegeneration and neural repair mechanisms[4]. Compared with the closely related isoform ROCK1, ROCK2 shares high homology within the kinase domain but exhibits distinct tissue distribution and isoform-specific biological functions, highlighting the importance of discriminating ROCK2-dependent signaling from broader ROCK activity in mechanistic studies[3][2]. For experimental applications, pharmacological inhibition of ROCK signaling is widely used to interrogate cytoskeletal regulation and disease-associated pathways, and the development of isoform-selective inhibitors has increased interest in defining ROCK2-specific functions with greater precision[2]>.

  • Subcellular Localization

    Cytoplasm; Cell membrane; Peripheral membrane protein; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Tissue_specificity:Expressed in the brain (at protein level)

  • Subunit

    Homodimer (By similarity). Interacts with PPP1R12A (PubMed:10579722, PubMed:19131646). Interacts with EP300 (PubMed:16574662). Interacts with CHORDC1 (PubMed:20230755). Interacts with BRCA2 (PubMed:21084279).

  • SwissProt ID

    O75116

  • Gene ID
    9475 [NCBI]
  • Synonyms

    p164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha

  • Research Field

    Cell Biology

[1]. Wikipedia.

[2]. Koch JC, et al. ROCK2 is a major regulator of axonal degeneration, neuronal death and axonal regeneration in the CNS. Cell Death Dis. 2014 May 15;5(5):e1225. [Content Brief]

[3]. ROCK2 gene information from NCBI.

[4]. Wikipedia.

[5]. Julian L, et al. Rho-associated coiled-coil containing kinases (ROCK): structure, regulation, and functions. Small GTPases. 2014;5:e29846. [Content Brief]

[6]. Loirand G. Rho Kinases in Health and Disease: From Basic Science to Translational Research. Pharmacol Rev. 2015;67(4):1074-1095.

View More

ROCK2 Antibody (YA1667) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg), using ROCK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ROCK2 Antibody (YA1667) Product Image
    1/16
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • ROCK2 Antibody (YA1667) Product Image
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg), using ROCK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • Experimental Validation Results for ROCK2 Antibody (YA1667)
  • ROCK2 Antibody (YA1667) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), A549 (lane 4(20μg), using ROCK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ROCK2 Antibody (YA1667) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ROCK2 Antibody (YA1667) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling ROCK2 with ROCK2 Antibody (HY-P81922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ROCK2 Antibody (HY-P81922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ROCK2 Antibody (YA1667) Product Image WB
  • ROCK2 Antibody (YA1667) Product Image ICC
  • ROCK2 Antibody (YA1667) Product Image ICC