Cyclophilin F Antibody (YA2602)(PBS only)

Cat. No.: HY-P82857A: Data Sheet User Guide for Antibodies
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Cyclophilin F Antibody (YA2602) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclophilin F.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cyclophilin F Antibody (YA2602) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclophilin F.

Host

Rabbit

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 18 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P30405

Gene ID

10105 [NCBI]

Immunogen

A synthetic peptide of human Cyclophilin F

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed:26387735). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed:26387735). In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed:22726440). Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (By similarity). Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (PubMed:19228691)

Subcellular Localization：Mitochondrion matrix

Isoforms & Post-Translational Modification：P30405 has 2 isomers: P30405-1: 22040 Da (predicted); P30405-2: 16541 Da (predicted).
Acetylated at Lys-167; deacetylated at Lys-167 by SIRT3

Subunit：Interacts with BCL2; the interaction is impaired by CsA (PubMed:19228691). Interacts with TP53; the association implicates preferentially tetrameric TP53, is induced by oxidative stress and is impaired by CsA (PubMed:22726440).

Research Field

Immunology

Synonyms

PPIF; CYP3; Peptidyl-prolyl cis-trans isomerase F; mitochondrial; PPIase F; Cyclophilin F; Rotamase F

Documentation

User Guide for Antibodies (1077 KB)