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ATP1A3 Antibody (YA2462)

(Synonyms: AHC2; Atp1a3; DYT12; RDP)
Customer Review

Based on 1 Customer Validation

ATP1A3 Antibody (YA2462) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1A3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATP1A3 Antibody (YA2462)
  • ATP1A3 Antibody (YA2462) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ATP1A3 Antibody (YA2462) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1A3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100-150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 112 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ATP1A3 aa1-58 aa1-58/1013.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP1A3 Antibody (YA2462)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2, 37℃, 15 mins), Rat brain (lane 3, 37℃, 15 mins), Mouse brain (lane 4, 65℃, 10 mins), Rat brain (lane 5, 65℃, 10 mins), Mouse brain (lane 6, 95℃, 10 mins), and Rat brain (lane 7, 95℃, 10 mins) using ATP1A3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    ATP1A3 is a This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    P13637 has 3 isomers: P13637-1: 111749 Da (predicted); P13637-2: 113134 Da (predicted); P13637-3: 113059 Da (predicted).

  • Subunit

    The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1

  • SwissProt ID

    P13637

  • Gene ID
    478 [NCBI]
  • Synonyms

    AHC2; Atp1a3; DYT12; RDP

  • Research Field

    Signal Transduction

ATP1A3 Antibody (YA2462) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2, 37℃, 15 mins), Rat brain (lane 3, 37℃, 15 mins), Mouse brain (lane 4, 65℃, 10 mins), Rat brain (lane 5, 65℃, 10 mins), Mouse brain (lane 6, 95℃, 10 mins), and Rat brain (lane 7, 95℃, 10 mins) using ATP1A3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ATP1A3 Antibody (YA2462) Product Image
    1/16
  • Experimental Validation Results for ATP1A3 Antibody (YA2462)
  • ATP1A3 Antibody (YA2462) Product Image
  • Experimental Validation Results for ATP1A3 Antibody (YA2462)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2, 37℃, 15 mins), Rat brain (lane 3, 37℃, 15 mins), Mouse brain (lane 4, 65℃, 10 mins), Rat brain (lane 5, 65℃, 10 mins), Mouse brain (lane 6, 95℃, 10 mins), and Rat brain (lane 7, 95℃, 10 mins) using ATP1A3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ATP1A3 Antibody (YA2462)
  • ATP1A3 Antibody (YA2462) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2, 37℃, 15 mins), Rat brain (lane 3, 37℃, 15 mins), Mouse brain (lane 4, 65℃, 10 mins), Rat brain (lane 5, 65℃, 10 mins), Mouse brain (lane 6, 95℃, 10 mins), and Rat brain (lane 7, 95℃, 10 mins) using ATP1A3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ATP1A3 Antibody (YA2462) Product Image WB