GCKR Antibody (YA8386)

Cat. No.: HY-P88702: Data Sheet User Guide for Antibodies
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GCKR Antibody (YA8386) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GCKR.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GCKR Antibody (YA8386) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GCKR.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 68.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q14397

Immunogen

Full length human recombinant protein of human GCKR produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-3000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed:23621087, PubMed:23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed:10456334). The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed:23621087, PubMed:23733961)

Subcellular Localization：Cytoplasm,Nucleus,Mitochondrion

Expression：
Tissue_Specificity: Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen

Isoforms & Post-Translational Modification：Q14397 has two isomers: Q14397-1: 68685 Da (predicted); Q14397-2: 16477 Da (predicted).

Subunit：Interacts (fructose 6-phosphate bound form) with GCK

Synonyms

FGQTL5; GKRP

Documentation

User Guide for Antibodies (1077 KB)