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VRK1 Antibody (YA7880)

(Synonyms: PCH1; PCH1A)
Customer Review

Based on 1 Customer Validation

VRK1 Antibody (YA7880) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VRK1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VRK1 Antibody (YA7880)
  • VRK1 Antibody (YA7880) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

VRK1 Antibody (YA7880) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VRK1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 45.3 kDa
Immunogen

Human recombinant protein fragment corresponding to amino acids 1-230 of human VRK1 produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VRK1 Antibody (YA7880)
    Western blot analysis of extracts from C6 (lane2(20μg), U87 (lane3(20μg), L929 (lane4(20μg) and HL-60 (lane5(20μg) using VRK1 Antibody (HY-P88196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    VRK1 is a nuclear Ser/Thr chromatin kinase that phosphorylates p53, histones, and DNA-damage-response proteins, linking cell proliferation with chromatin stability[1][2][3]. Mechanistically, VRK1 supports cell-cycle entry, G2/M chromatin condensation, and non-homologous end joining through γH2AX, NBS1, and 53BP1 foci formation after ionizing radiation or chemotherapy[1][2]. In p53 signaling, VRK1 phosphorylates p53 at Thr18, stabilizes transcriptionally active p53, and participates in a regulatory loop that modulates DNA-damage responses[3]. In disease models, VRK1 overexpression associates with tumor progression and resistance to genotoxic treatments, and VRK1 loss reduces 53BP1 repair foci after ionizing radiation or doxorubicin in breast cancer cells[1][4]. Compared with related isoforms, VRK1 is mostly nuclear, whereas VRK2A anchors to endoplasmic-reticulum and mitochondrial membranes and VRK2B distributes in cytosol and nucleus, suggesting localization-dependent substrate access[5]. For experimental applications, kinase-inhibitor profiling shows VRK1 and VRK2 have different sensitivity patterns, providing a framework for designing VRK1-selective inhibitors with reduced VRK2 cross-inhibition[6].

  • Subcellular Localization

    Nucleus,Cytoplasm,Nucleus, Cajal body

  • Expression


    Tissue_Specificity: Widely expressed. Highly expressed in fetal liver, testis and thymus

  • Isoforms & Post-Translational Modification

    Q99986: 396 amino acids, molecular weight 45476 Da.

  • Subunit

    Interacts with HDAC1, KAT2B, SETDB1, KDM3A and KDM4A (PubMed:37179361)

  • SwissProt ID

    Q99986

  • Synonyms

    PCH1; PCH1A

References

[1]. Campillo-Marcos I, et al. The human VRK1 chromatin kinase in cancer biology. Cancer Lett. 2021 Apr 10;503:117-128. [Content Brief]

[2]. Sanz-García M, et al. Vaccinia-related kinase 1 (VRK1) is an upstream nucleosomal kinase required for the assembly of 53BP1 foci in response to ionizing radiation-induced DNA damage. J Biol Chem. 2012 Jul 6;287(28):23757-68. [Content Brief]

[3]. Vega FM, et al. p53 Stabilization and accumulation induced by human vaccinia-related kinase 1. Mol Cell Biol. 2004 Dec;24(23):10366-80. [Content Brief]

[4]. Salzano M, et al. Vaccinia-related kinase 1 (VRK1) confers resistance to DNA-damaging agents in human breast cancer by affecting DNA damage response. Oncotarget. 2014 Apr 15;5(7):1770-8. [Content Brief]

[5]. Blanco S, et al. The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines. FEBS J. 2006 Jun;273(11):2487-504. [Content Brief]

[6]. Vázquez-Cedeira M, et al. Differential inhibitor sensitivity between human kinases VRK1 and VRK2. PLoS One. 2011;6(8):e23235. [Content Brief]

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VRK1 Antibody (YA7880) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane2(20μg), U87 (lane3(20μg), L929 (lane4(20μg) and HL-60 (lane5(20μg) using VRK1 Antibody (HY-P88196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • VRK1 Antibody (YA7880) Product Image
    1/16
  • Experimental Validation Results for VRK1 Antibody (YA7880)
  • VRK1 Antibody (YA7880) Product Image
  • Experimental Validation Results for VRK1 Antibody (YA7880)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane2(20μg), U87 (lane3(20μg), L929 (lane4(20μg) and HL-60 (lane5(20μg) using VRK1 Antibody (HY-P88196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VRK1 Antibody (YA7880)
  • VRK1 Antibody (YA7880) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane2(20μg), U87 (lane3(20μg), L929 (lane4(20μg) and HL-60 (lane5(20μg) using VRK1 Antibody (HY-P88196). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • VRK1 Antibody (YA7880) Product Image WB