Scramblase 1 Antibody (YA2419)
(Synonyms: MmTRA1a; MmTRA1b; Nor1; PLSCR 1; Scramblase1; Tra1; Tra1a; Tra1b; Tras1; Tras2)
Based on 1 Customer Validation
Scramblase 1 Antibody (YA2419) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Scramblase 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC, IP
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50-1:200 | 1:50-1:100 | 1:50 |
Product Details
Scramblase 1 Antibody (YA2419) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Scramblase 1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
A synthesized peptide derived from human Scramblase 1 aa20-55.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Scramblase 1 catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membrane resulting in collapse of the phospholipid asymmetry which leads to phosphatidylserine externalization on the cell surface. Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with TRPC5. Also exhibits magnesium-dependent nuclease activity against double-stranded DNA and RNA but not single-stranded DNA and can enhance DNA decatenation mediated by TOP2A. Negatively regulates FcR-mediated phagocytosis in differentiated macrophages. May contribute to cytokine-regulated cell proliferation and differentiation. May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. Inhibits the functions of viral transactivators, including human T-cell leukemia virus (HTLV)-1 protein Tax, human immunodeficiency virus (HIV)-1 Tat, human hepatitis B virus (HBV) HBx, Epstein-Barr virus (EBV) BZLF1 and human cytomegalovirus IE1 and IE2 proteins through direct interactions. Also mediates the inhibition of influenza virus infection by preventing nuclear import of the viral nucleoprotein/NP. Plays a crucial role as a defense factor against SARS-CoV-2 independently of its scramblase activity by directly targeting nascent viral vesicles to prevent virus-membrane fusion and the release of viral RNA into the host-cell cytosol; (Microbial infection) Acts as an attachment receptor for HCV[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].
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Subcellular Localization
Cell membrane; Single-pass type II membrane protein; Cell membrane; Lipid-anchor; Cytoplasmic side; Nucleus; Cytoplasm; Cytoplasm, perinuclear region
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Expression
Tissue_specificity:Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle
Induction: (Microbial infection) Induced by IFNB1/IFN-beta in response to a viral infection; Up-regulated during PMA-induced differentiation of the monocytic cell line THP-1 -
Isoforms & Post-Translational Modification
O15162 has 2 isomers: O15162-1: 35049 Da (predicted); O15162-2: 26825 Da (predicted).
Phosphorylation at Thr-161 by PKC/PKCD increases its phospholipid scramblase activity during both cell stimulation and apoptosis (PubMed:10770950). Phosphorylated by OXSR1 in the presence of RELT;Palmitoylation is required for its phospholipid scramblase activity (PubMed:9572851). Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus (PubMed:12564925) -
Subunit
Forms homooligomers in the presence of calcium (PubMed:24648509). Interacts with ABL (PubMed:11390389). Interacts with RELT, RELL1 and RELL2 (PubMed:22052202). Interacts with OXSR1 in the presence of RELT (PubMed:22052202).
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SwissProt ID
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Synonyms
MmTRA1a; MmTRA1b; Nor1; PLSCR 1; Scramblase1; Tra1; Tra1a; Tra1b; Tras1; Tras2
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Research Field
Microbiology
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