2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. NSE Antibody (YA1005)

NSE Antibody (YA1005) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NSE.

For research use only. We do not sell to patients.

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20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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Description

NSE Antibody (YA1005) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NSE.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 47 kDa;
Observed band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide derived from human NSE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from U-87MG (lane 1, 15 μg), IMR-32 (lane 2, 15 μg), PC-12 (lane 3, 15 μg), Neuro-2a (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using NSE Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity)
Subcellular Localization：Cytoplasm; Cell membrane
Expression：
Tissue_specificity:α/α homodimers are expressed in embryos and most adult tissues. α/β heterodimers and β/β homodimers are present in skeletal muscle, while α/γ heterodimers and γ/γ homodimers are present in neurons.

Induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease; (Microbial infection) Activated by human cytomegalovirus (HCMV) UL38 in order to provide the virus with glycosyl building blocks
Isoforms & Post-Translational Modification：P09104 has 2 isomers: P09104-1: 47269 Da (predicted); P09104-2: 42707 Da (predicted).
Subunit：Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific
Database

Entrez Gene: 2026 Human ; 13807 Mouse ; 24334 Rat

SwissProt: P09104 Human ; P17183 Mouse ; P07323 Rat

OMIM: 131360 Human

Research Field

Signal Transduction
Synonyms
ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
Documentation
User Guide for Antibodies (1077 KB)

NSE Antibody (YA1005) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NSE Antibody (YA1005)
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HY-P81290A
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