MARK2 Antibody (YA7725)

Cat. No.: HY-P88040: Data Sheet User Guide for Antibodies
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MARK2 Antibody (YA7725) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

MARK2 Antibody (YA7725) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK2.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 88 kDa;

Species Reactivity

Human Predicted Reactivity: Human,Mouse,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q7KZI7

Immunogen

Recombinant human MARK2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
FC FC: Flow Cytometry	1:50-100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 10mM TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells

Subcellular Localization：Cell membrane,Cytoplasm,Lateral cell membrane,Cytoplasm, cytoskeleton,Cell projection, dendrite,Cytoplasm

Expression：
Tissue_Specificity: High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney

Isoforms & Post-Translational Modification：Q7KZI7 has 16 isomers: Q7KZI7-1: 87911 Da (predicted); Q7KZI7-2: 78851 Da (predicted); Q7KZI7-3: 83184 Da (predicted); Q7KZI7-4: 81280 Da (predicted); Q7KZI7-5: 80647 Da (predicted); Q7KZI7-6: 84333 Da (predicted); Q7KZI7-7: 77702 Da (predicted); Q7KZI7-8: 86762 Da (predicted); Q7KZI7-9: 82429 Da (predicted); Q7KZI7-10: 77069 Da (predicted); Q7KZI7-11: 86129 Da (predicted). Q7KZI7-12: 82551 Da (predicted); Q7KZI7-13: 77631 Da (predicted); Q7KZI7-14: 83113 Da (predicted); Q7KZI7-15: 79427 Da (predicted); Q7KZI7-16: 81209 Da (predicted).
Autophosphorylated

Subunit：Homodimer

Synonyms

EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/baf3-cell.html [Content Brief]