CCR2 Antibody (YA4008)(PBS only)
(Synonyms: CCR2; CKR2; CCR-2; CCR2A; CCR2B; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2)CCR2 Antibody (YA4008) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCR2.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
CCR2 Antibody (YA4008) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCR2.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 41.9 kDa
Purified recombinant fragment of human CD192 expressed in E. Coli.
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CCR2 is a CC chemokine receptor that mediates MCP-1/CCL2-dependent monocyte chemotaxis, receptor binding, and ligand internalization in inflammatory and vascular contexts[1][2]. Mechanistically, the CCR2 amino-terminal domain is necessary and sufficient for high-affinity MCP-1 binding, while tyrosine sulfation enhances CCR2 interaction with monomeric and dimeric MCP-1 and supports leukocyte recruitment[1][3]. In human monocytes, LDL increases CCR2 expression and strengthens MCP-1 chemotactic responses, linking CCR2 signaling to monocyte recruitment during atherogenesis[4]. In disease models, leukocyte-derived CCR2 is critical for angiotensin II-accelerated atherosclerosis and aneurysm formation in hypercholesterolemic mice[5]. CCR2 also mediates MCP-1 binding and internalization along brain microvessels, supporting its use in blood-brain barrier and neuroinflammation models[6]. Compared with related isoforms, CCR2A and CCR2B show cell-specific distribution in idiopathic inflammatory myopathies, where CCR2A appears in vessel walls and mononuclear cells, while CCR2B appears in satellite cells, neuromuscular junction domains, and regenerative fibers[7]. For experimental applications, MCP-1 binds and activates CCR2B as a monomer, and CCR2-blocking antibodies reduce myeloma-cell migration toward bone marrow stromal chemokines[8][9].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Expression by monocytes and IL2-activated NK cells (PubMed:9058802) . Highly expressed on CD14+/CD16- monocytes, and weakly expressed on CD14+/CD16+ monocytes, type 2 dendritic cells (DCs) , and plasmacytoid dendritic cells (protein level) (PubMed:38157855) .
Induction:Up-regulated by CREB3 (PubMed:18587271) . In NK cells, induced by IL2 (PubMed:9058802) -
Subunit
Interacts with ARRB1 (PubMed:9501202). Interacts (via extracellular N-terminal region) with beta-defensin DEFB106A/DEFB106B; this interaction may preferentially require specific tyrosine sulfation on CCR2 (PubMed:23938203). Interacts with NUP85; the interaction is required for CCR2 clusters formation on the cell membrane and CCR2 signaling (PubMed:15995708, PubMed:25283965)
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SwissProt ID
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Synonyms
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Documentation
References
[1]. Monteclaro FS, et al. The amino-terminal domain of CCR2 is both necessary and sufficient for high affinity binding of monocyte chemoattractant protein 1. Receptor activation by a pseudo-tethered ligand. J Biol Chem. 1997 Sep 12;272(37):23186-90. [Content Brief]
[2]. Preobrazhensky AA, et al. Monocyte chemotactic protein-1 receptor CCR2B is a glycoprotein that has tyrosine sulfation in a conserved extracellular N-terminal region. J Immunol. 2000 Nov 1;165(9):5295-303. [Content Brief]
[3]. Tan JHY, et al. Tyrosine sulfation of chemokine receptor CCR2 enhances interactions with both monomeric and dimeric forms of the chemokine monocyte chemoattractant protein-1 (MCP-1). J Biol Chem. 2013 Apr 5;288(14):10024-10034. [Content Brief]
[4]. Han KH, et al. Chemokine receptor CCR2 expression and monocyte chemoattractant protein-1-mediated chemotaxis in human monocytes. A regulatory role for plasma LDL. Arterioscler Thromb Vasc Biol. 1998 Dec;18(12):1983-91. [Content Brief]
[5]. Ishibashi M, et al. Bone marrow-derived monocyte chemoattractant protein-1 receptor CCR2 is critical in angiotensin II-induced acceleration of atherosclerosis and aneurysm formation in hypercholesterolemic mice. Arterioscler Thromb Vasc Biol. 2004 Nov;24(11):e174-8. [Content Brief]
[6]. Dzenko KA, et al. The chemokine receptor CCR2 mediates the binding and internalization of monocyte chemoattractant protein-1 along brain microvessels. J Neurosci. 2001 Dec 1;21(23):9214-23. [Content Brief]
[7]. Bartoli C, et al. CCR2A and CCR2B, the two isoforms of the monocyte chemoattractant protein-1 receptor are up-regulated and expressed by different cell subsets in idiopathic inflammatory myopathies. Acta Neuropathol. 2001 Oct;102(4):385-92. [Content Brief]
[8]. Paavola CD, et al. Monomeric monocyte chemoattractant protein-1 (MCP-1) binds and activates the MCP-1 receptor CCR2B. J Biol Chem. 1998 Dec 11;273(50):33157-65. [Content Brief]
[9]. Vande Broek I, et al. Chemokine receptor CCR2 is expressed by human multiple myeloma cells and mediates migration to bone marrow stromal cell-produced monocyte chemotactic proteins MCP-1, -2 and -3. Br J Cancer. 2003 Mar 24;88(6):855-62. [Content Brief]
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