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Cat. No.: HY-P82234A

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ACOX1 Antibody (YA1979)(PBS only)

Name: ACOX1 Antibody (YA1979)(PBS only)
Brand: MedChemExpress
SKU: HY-P82234A
Availability: PreOrder

(Synonyms: AOX; Acox; Paox; D130055E20Rik)

ACOX1 Antibody (YA1979) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACOX1.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	IP IP: Immunoprecipitation
Dilution Ratio	1:500-1:1000	1:50-1:200	1:20

Description

ACOX1 Antibody (YA1979) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACOX1.

Host Rabbit
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 74,48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 75 kDa

Immunogen

Recombinant protein of mouse ACOX1 aa1-270.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using ACOX1 Antibody (HY-P82234) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Function

ACOX1 is involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids. Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)); Shows highest activity against medium-chain fatty acyl-CoAs. Shows optimum activity with a chain lengtH2O2 10 carbons (decanoyl-CoA) in vitro; Is active against a much broader range of substrates and shows activity towards long-chain acyl-CoAs
Subcellular Localization

Peroxisome
Expression

Tissue_specificity:Highest levels of isoform 1 are found in liver and kidney while highest levels of isoform 2 are found in white adipose tissue. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 is expressed at higher levels in brain, heart, lung, muscle, white adipose tissue and testis
Isoforms & Post-Translational Modification

Q9R0H0 has 2 isomers: Q9R0H0-1: 74649 Da (predicted); Q9R0H0-2: 74718 Da (predicted).
Subunit

Homodimer (By similarity). Interacts with LONP2 (By similarity)
SwissProt ID

Q9R0H0
Gene ID

51 [NCBI]
Synonyms

AOX; Acox; Paox; D130055E20Rik
Research Field

Signal Transduction