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PDGF B Antibody (YA1556)(PBS only)

(Synonyms: PDGFB; PDGF2; SIS; Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin)

PDGF B Antibody (YA1556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDGF B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • PDGF B Antibody (YA1556)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

PDGF B Antibody (YA1556) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDGF B.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Immunogen

A synthetic peptide of human PDGF B

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
    Western blot analysis of extracts from MCF-7(lane 2(20μg), SH-SY5Y(lane 3(20μg), HUVEC(lane 4(20μg) and Mouse Brain(lane 5(20μg) using PDGF B Antibody (HY-P81811). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PDGF B is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA[1].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed at high levels in the heart, brain (sustantia nigra) , placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus) , skeletal muscle, kidney and lung

  • Isoforms & Post-Translational Modification

    P01127 has 2 isomers: P01127-1: 27283 Da (predicted); P01127-2: 25502 Da (predicted).

  • Subunit

    Antiparallel homodimer; disulfide-linked. Antiparallel heterodimer with PDGFA; disulfide-linked. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB.

  • SwissProt ID

    P01127

  • Gene ID
    5155 [NCBI]
  • Synonyms

    PDGFB; PDGF2; SIS; Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin

  • Research Field

    Cardiovascular

PDGF B Antibody (YA1556)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg), SH-SY5Y(lane 3(20μg), HUVEC(lane 4(20μg) and Mouse Brain(lane 5(20μg) using PDGF B Antibody (HY-P81811). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PDGF B Antibody (YA1556)(PBS only) Product Image
    1/16
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • PDGF B Antibody (YA1556)(PBS only) Product Image
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg), SH-SY5Y(lane 3(20μg), HUVEC(lane 4(20μg) and Mouse Brain(lane 5(20μg) using PDGF B Antibody (HY-P81811). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • Experimental Validation Results for PDGF B Antibody (YA1556)(PBS only)
  • PDGF B Antibody (YA1556)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20μg), SH-SY5Y(lane 3(20μg), HUVEC(lane 4(20μg) and Mouse Brain(lane 5(20μg) using PDGF B Antibody (HY-P81811). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PDGF B Antibody (YA1556)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PDGF B Antibody (YA1556)(PBS only) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using PDGF B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PDGF B Antibody (YA1556)(PBS only) Product Image WB
  • PDGF B Antibody (YA1556)(PBS only) Product Image IHC-P
  • PDGF B Antibody (YA1556)(PBS only) Product Image IHC-P