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Phospho-Synapsin I (Ser9) Antibody (YA1864)

(Synonyms: SYN1; Synapsin-1; Brain protein 4.1; Synapsin I)
Customer Review

Based on 1 Customer Validation

Phospho-Synapsin I (Ser9) Antibody (YA1864) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Synapsin I (Ser9).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Phospho-Synapsin I (Ser9) Antibody (YA1864) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Synapsin I (Ser9).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 74 kDa
Species Reactivity Database
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser9 of human Synapsin I

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg) using Phospho-Synapsin I (Ser9) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    Synapsin I is a Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal. Also involved in the regulation of axon outgrowth and synaptogenesis. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level[1][2].

  • Subcellular Localization

    Synapse; Golgi apparatus; Presynapse; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle

  • Isoforms & Post-Translational Modification

    P17600 has 2 isomers: P17600-1: 74111 Da (predicted); P17600-2: 70033 Da (predicted).
    Substrate of different protein kinases. Phosphorylated by CaMK2 and MAPK1 (PubMed:21441247). Phosphorylation, including phosphorylation at Ser-9, promotes synapsin-1 dissociation from synaptic vesicles, regulates its rate of dispersion, and controls the kinetics of vesicle pool turnover and neurotransmitter release (By similarity) (PubMed:21441247)

  • Subunit

    Homodimer (By similarity). Can form oligomers with SYN2 (PubMed:23406870). Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity). Isoform Ib interacts with PRNP (By similarity)

  • SwissProt ID

    P17600

  • Gene ID
    6853 [NCBI]
  • Synonyms

    SYN1; Synapsin-1; Brain protein 4.1; Synapsin I

  • Research Field

    Neuroscience

Phospho-Synapsin I (Ser9) Antibody (YA1864) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg) using Phospho-Synapsin I (Ser9) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    1/16
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg) using Phospho-Synapsin I (Ser9) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Experimental Validation Results for Phospho-Synapsin I (Ser9) Antibody (YA1864)
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg) using Phospho-Synapsin I (Ser9) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82119, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated overnight at 4°C with the primary antibody (HY-P82119,1:50 dilution). Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Brain tissue using Phospho-Synapsin I (Ser9) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82119, 1:50 dilution) at room temperature for Leave overnight at 4°C and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image WB
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image IHC-P
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image IHC-P
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image IHC-P
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image IHC-P
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image IHC-P
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image IHC-P
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image mIHC
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image mIHC
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image mIHC
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image mIHC
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image mIHC
  • Phospho-Synapsin I (Ser9) Antibody (YA1864) Product Image mIHC