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Elongation Factor 1A2 Antibody (YA2879)

(Synonyms: HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2)
Customer Review

Based on 1 Customer Validation

Elongation Factor 1A2 Antibody (YA2879) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation Factor 1A2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Elongation Factor 1A2 Antibody (YA2879)
  • Elongation Factor 1A2 Antibody (YA2879) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20-1:50

Product Details

Description

Elongation Factor 1A2 Antibody (YA2879) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation Factor 1A2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human EEF1A2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Elongation Factor 1A2 Antibody (YA2879)
    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HepG2 (lane 3), and Mouse heart (lane 4) using Elongation Factor 1A2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Elongation Factor 1A2 is a Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation

  • Subcellular Localization

    Endoplasmic reticulum membrane

  • Expression


    Tissue_specificity:Brain, heart, and skeletal muscle

  • Subunit

    Homodimer; arranged in a 'head to tail' dimer configuration

  • SwissProt ID

    Q05639

  • Gene ID
    1917 [NCBI]
  • Synonyms

    HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2

  • Research Field

    Epigenetics and Nuclear Signaling

Elongation Factor 1A2 Antibody (YA2879) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HepG2 (lane 3), and Mouse heart (lane 4) using Elongation Factor 1A2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Elongation Factor 1A2 Antibody (YA2879) Product Image
    1/16
  • Experimental Validation Results for Elongation Factor 1A2 Antibody (YA2879)
  • Elongation Factor 1A2 Antibody (YA2879) Product Image
  • Experimental Validation Results for Elongation Factor 1A2 Antibody (YA2879)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HepG2 (lane 3), and Mouse heart (lane 4) using Elongation Factor 1A2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Elongation Factor 1A2 Antibody (YA2879)
  • Elongation Factor 1A2 Antibody (YA2879) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HepG2 (lane 3), and Mouse heart (lane 4) using Elongation Factor 1A2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Elongation Factor 1A2 Antibody (YA2879) Product Image WB