CD133 Antibody (YA6315)
(Synonyms: PROM1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133)
Based on 1 Customer Validation
CD133 Antibody (YA6315) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD133.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:20-100 | 1:1000-5000 | 1:200-1000 | 1:5000-20000 | 1:50-200 |
Product Details
CD133 Antibody (YA6315) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD133.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 133 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 97 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD133 may play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner[1][2].
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Subcellular Localization
Apical cell membrane; Multi-pass membrane protein; Cell projection, microvillus membrane; Multi-pass membrane protein; Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum; Endoplasmic reticulum-Golgi intermediate compartment
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Expression
Tissue_specificity:Isomer 1 is selectively expressed on CD34+ hematopoietic stem cells and progenitor cells in human bone marrow, fetal liver, umbilical cord blood, and human peripheral blood. Isomer 1 is not detected in other blood cells. Isomer 1 is also expressed in a variety of non-lymphoid tissues, including the retina, pancreas, placenta, kidney, liver, lung, brain, and heart. In saliva, isomer 1 exists as small membrane granules. Isomer 2 is primarily expressed in fetal liver, skeletal muscle, kidney, and heart, as well as in human pancreas, kidney, liver, lung, and placenta. Isomer 2 is highly expressed in fetal liver, lowly expressed in bone marrow, and almost undetectable in peripheral blood. Isomer 2 is expressed on hematopoietic stem cells and epidermal basal cells (protein level) . In the human retina, isomer 2 is expressed by rod cells and cone cells (protein level) . -
Isoforms & Post-Translational Modification
O43490 has 7 isomers: O43490-1: 97202 Da (predicted); O43490-2: 96251 Da (predicted); O43490-3: 93270 Da (predicted); O43490-4: 93654 Da (predicted); O43490-5: 92741 Da (predicted); O43490-6: 93692 Da (predicted); O43490-7: 94605 Da (predicted).
Isoform 1 and isoform 2 are glycosylated;Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis -
Subunit
Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B
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SwissProt ID
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Synonyms
PROM1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133
Documentation
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