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AKR1C1 Antibody (YA4180)

(Synonyms: C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD)
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Based on 1 Customer Validation

AKR1C1 Antibody (YA4180) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AKR1C1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rabbit

  • Formulation:

    1.Supplied in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.
    2.Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • AKR1C1 Antibody (YA4180) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:200-1:400 1:200-1:400

Product Details

Description

AKR1C1 Antibody (YA4180) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AKR1C1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rabbit
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Immunogen

Purified fusion protein with human AKR1C1 aa 1-323.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.
    2.Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    Western blot analysis of extracts from Mouse liver(lane 1(40μg) ),HepG2(lane 2(40μg) ),A549(lane 3(40μg) )and Hela(lane 4(40μg) ) using AKR1C1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    Western blot analysis of extracts from HepG2(lane2(20μg), U87-MG(lane3(20μg), Hela(lane4(20μg) and A431(lane5(20μg) using AKR1C1 Antibody (HY-P84483). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    Flow cytometric analysis of 1X10^6 Hela cells labeling AKR1C1 Antibody(red).Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    Flow cytometric analysis of 1X106 HeLa cells labeling AKR1C1 Antibody(HY-P84483, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    AKR1C1 is a Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids[1][2][3][4].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Tissue_specificity:It was expressed in all tissues tested, including the liver, prostate, testes, adrenal glands, brain, uterus, breast, and keratinocytes. The highest levels were found in the liver, breast, and brain.

  • Subunit

    Monomer

  • SwissProt ID

    Q04828

  • Gene ID
    1645 [NCBI]
  • Synonyms

    C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD

AKR1C1 Antibody (YA4180) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse liver(lane 1(40μg) ),HepG2(lane 2(40μg) ),A549(lane 3(40μg) )and Hela(lane 4(40μg) ) using AKR1C1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane2(20μg), U87-MG(lane3(20μg), Hela(lane4(20μg) and A431(lane5(20μg) using AKR1C1 Antibody (HY-P84483). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling AKR1C1 Antibody(red).Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling AKR1C1 Antibody(HY-P84483, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • AKR1C1 Antibody (YA4180) Product Image
    1/16
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • AKR1C1 Antibody (YA4180) Product Image
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse liver(lane 1(40μg) ),HepG2(lane 2(40μg) ),A549(lane 3(40μg) )and Hela(lane 4(40μg) ) using AKR1C1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane2(20μg), U87-MG(lane3(20μg), Hela(lane4(20μg) and A431(lane5(20μg) using AKR1C1 Antibody (HY-P84483). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling AKR1C1 Antibody(red).Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling AKR1C1 Antibody(HY-P84483, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • Experimental Validation Results for AKR1C1 Antibody (YA4180)
  • AKR1C1 Antibody (YA4180) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse liver(lane 1(40μg) ),HepG2(lane 2(40μg) ),A549(lane 3(40μg) )and Hela(lane 4(40μg) ) using AKR1C1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • AKR1C1 Antibody (YA4180) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane2(20μg), U87-MG(lane3(20μg), Hela(lane4(20μg) and A431(lane5(20μg) using AKR1C1 Antibody (HY-P84483). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • AKR1C1 Antibody (YA4180) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling AKR1C1 Antibody(red).Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • AKR1C1 Antibody (YA4180) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling AKR1C1 Antibody(HY-P84483, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • AKR1C1 Antibody (YA4180) Product Image WB
  • AKR1C1 Antibody (YA4180) Product Image WB
  • AKR1C1 Antibody (YA4180) Product Image FC
  • AKR1C1 Antibody (YA4180) Product Image FC