HLA-DR Antibody (YA6179)
(Synonyms: HLA-DRA1, HLA-DRA, MHC class II antigen DRA)
Based on 1 Customer Validation
HLA-DR Antibody (YA6179) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HLA-DR.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:4000 | 1:1000-1:5000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
HLA-DR Antibody (YA6179) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HLA-DR.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 37 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 29 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HLA-DR is a heterodimeric transmembrane glycoprotein composed of two non-covalently linked chains: an α chain and a β chain. HLA-DR is primarily expressed on the surface of professional antigen-presenting cells (APCs). It functions in antigen presentation and coordinates T-cell activation and differentiation, thereby facilitating the generation of an effective immune response.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Early endosome membrane; Single-pass type I membrane protein; Late endosome membrane; Single-pass type I membrane protein; Lysosome membrane; Single-pass type I membrane protein; Autolysosome membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:Expressed in professional APCs: macrophages, dendritic cells and B cells (at protein level). Expressed in thymic epithelial cells (at protein level)
Induction:Up-regulated in dendritic cells upon maturation -
Subunit
Heterotrimer that consists of an alpha chain HLA-DRA, a beta chain HLA-DRB and a peptide (peptide-MHCII)
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SwissProt ID
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Synonyms
HLA-DRA1, HLA-DRA, MHC class II antigen DRA
Documentation
References
[1]. Shams H, et al. Characterization of a Mycobacterium tuberculosis peptide that is recognized by human CD4+ and CD8+ T cells in the context of multiple HLA alleles. J Immunol. 2004 Aug 1;173(3):1966-77. [Content Brief]
[2]. Röhn TA, et al. Upregulation of the CLIP self peptide on mature dendritic cells antagonizes T helper type 1 polarization. Nat Immunol. 2004 Sep;5(9):909-18. [Content Brief]
[3]. Deng L, et al. Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor. Nat Immunol. 2007 Apr;8(4):398-408. [Content Brief]
[4]. Kwok WW, et al. Frequency of epitope-specific naive CD4(+) T cells correlates with immunodominance in the human memory repertoire. J Immunol. 2012 Mar 15;188(6):2537-44. [Content Brief]
[5]. Scally SW, et al. A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis. J Exp Med. 2013 Nov 18;210(12):2569-82. [Content Brief]
[6]. Muehling LM, et al. Circulating Memory CD4+ T Cells Target Conserved Epitopes of Rhinovirus Capsid Proteins and Respond Rapidly to Experimental Infection in Humans. J Immunol. 2016 Oct 15;197(8):3214-3224. [Content Brief]
[7]. Galperin M, et al. CD4(+) T cell-mediated HLA class II cross-restriction in HIV controllers. Sci Immunol. 2018 Jun 8;3(24):. [Content Brief]
[8]. Abelin JG, et al. Defining HLA-II Ligand Processing and Binding Rules with Mass Spectrometry Enhances Cancer Epitope Prediction. Immunity. 2019 Oct 15;51(4):766-779.e17. [Content Brief]
[9]. Stern LJ, et al. Crystal structure of the human class II MHC protein HLA-DR1 complexed with an influenza virus peptide. Nature. 1994 Mar 17;368(6468):215-21. [Content Brief]
[10]. Kropshofer H, et al. HLA-DM acts as a molecular chaperone and rescues empty HLA-DR molecules at lysosomal pH. Immunity. 1997 Mar;6(3):293-302. [Content Brief]
[11]. Schmid D, et al. Antigen-loading compartments for major histocompatibility complex class II molecules continuously receive input from autophagosomes. Immunity. 2007 Jan;26(1):79-92. [Content Brief]
[12]. Adamopoulou E, et al. Exploring the MHC-peptide matrix of central tolerance in the human thymus. Nat Commun. 2013;4:2039. [Content Brief]
[13]. Kim A, et al. Divergent paths for the selection of immunodominant epitopes from distinct antigenic sources. Nat Commun. 2014 Nov 21;5:5369. [Content Brief]
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