Radixin Antibody (YA1810)(PBS only)

Art. -Nr.: HY-P82065A: Data Sheet User Guide for Antibodies
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Technical Support

Radixin Antibody (YA1810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Radixin.

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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

Radixin Antibody (YA1810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Radixin.

Host

Rabbit

Molekulargewicht

Predicted band size: 69 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35241

Gene ID

5962 [NCBI]

Immunogen

A synthetic peptide of human Radixin aa400-450.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:20-1:50
FC FC: Flow Cytometry	1:50-1:100

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane

Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cytoskeleton; Cleavage furrow; Cell projection, microvillus; Cell projection, stereocilium

Isoforms & Post-Translational Modification：P35241 has 5 isomers: P35241-1: 68564 Da (predicted); P35241-2: 29668 Da (predicted); P35241-3: 23413 Da (predicted); P35241-4: 52679 Da (predicted); P35241-5: 71049 Da (predicted).
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity)

Subunit：Binds NHERF1 (PubMed:9430655). Interacts with NHERF1, NHERF2, LAYN, MME/NEP and ICAM2. Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain). Interacts (via FERM domain) with SPN/CD43 cytoplasmic tail (By similarity). Interacts with CD44 (By similarity). Interacts with CLIC5; may work together in a complex which also includes EZR and MYO6 to stabilize linkages between the plasma membrane and subjacent actin cytoskeleton at the base of stereocilia (By similarity)

Research Field

Signal Transduction

Synonyms

RDX; Radixin

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/mcf7-cell.html [Content Brief]