KAT6A Antibody (YA6622)

(Synonyms: Histone acetyltransferase KAT6A EC:2.3.1.48 MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3 (MYST-3) Monocytic leukemia zinc finger protein Runt-related transcription factor-binding protein 2 Zinc finger protein 220 KAT6A MOZ MYST3 RUNXBP2 Histone acetyltransferase KAT6A EC:2.3.1.48 MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3 (MYST-3) Monocytic leukemia zinc finger protein Runt-related transcription factor-binding protein 2 Zinc finger protein 220 KAT6A MOZ MYST3 RUNXBP2 ZNF220 )
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Based on 1 publication(s) in Google Scholar

KAT6A Antibody (YA6622) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KAT6A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    ICC/IF, IHC-P, FC, ChIP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated

Publications Citing Use of MedChemExpress (MCE) KAT6A Antibody (YA6622)

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Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ChIP Info
ChIP: Chromatin Immunoprecipitation
Dilution Ratio 1:500 1:2000-1:20000 1:200-1:1000 Use 5 μg for 25 μg of chromatin.

Product Details

Description

KAT6A Antibody (YA6622) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KAT6A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 225 kDa
Immunogen

Recombinant protein within human KAT6A aa 785-1,034.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719229

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer‌ tissue using KAT6A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86929, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer‌ tissue using KAT6A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86929, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinoma tissue using KAT6A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86929, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using KAT6A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86929, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using KAT6A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86929, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using KAT6A antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P86929, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Breast Cancer tissue using KAT6A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86929, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Breast Cancer tissue using KAT6A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86929, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Breast Cancer tissue using KAT6A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86929, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using KAT6A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86929, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using KAT6A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86929, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for KAT6A Antibody (YA6622)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using KAT6A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P86929, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    KAT6A is a Histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. May act as a transcriptional coactivator for RUNX1 and RUNX2. Acetylates p53/TP53 at 'Lys-120' and 'Lys-382' and controls its transcriptional activity via association with PML

  • Subcellular Localization

    Nucleus,Nucleus, nucleolus,Nucleus, nucleoplasm,Nucleus, PML body

  • Isoforms & Post-Translational Modification

    Q92794: 2004 amino acids, molecular weight 225028 Da.

  • Subunit

    Component of the MOZ/MORF complex composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3

  • SwissProt ID

    Q92794

  • Gene ID
  • Synonyms

    Histone acetyltransferase KAT6A EC:2.3.1.48 MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3 (MYST-3) Monocytic leukemia zinc finger protein Runt-related transcription factor-binding protein 2 Zinc finger protein 220 KAT6A MOZ MYST3 RUNXBP2 Histone acetyltransferase KAT6A EC:2.3.1.48 MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3 (MYST-3) Monocytic leukemia zinc finger protein Runt-related transcription factor-binding protein 2 Zinc finger protein 220 KAT6A MOZ MYST3 RUNXBP2 ZNF220

KAT6A Antibody (YA6622) Related Classifications

MOQ
Minimum order quantity
100 mg

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