RAB7L1 Antibody
(Synonyms: RAB7L1, RAB29, Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29)RAB7L1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RAB7L1.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
RAB7L1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RAB7L1.
-
Host Rabbit
-
Clonality Polyclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 23 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 23 kDa
Synthetic peptide corresponding to the center region of human RAB7L1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
RAB7L1 is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB29 is essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity. Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2. Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.typhi) epithelial cell infection[1][2][3][4].
-
Subcellular Localization
Cell membrane; Cytoplasm; Cytoplasm, perinuclear region; Golgi apparatus; Golgi apparatus membrane; Golgi apparatus, trans-Golgi network; Vacuole; Cytoplasm, cytoskeleton
-
Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
RAB7L1 has 3 isoforms, O14966-1: amino acid length is 203, molecular weight is 23155 Da (predicted); O14966-2: amino acid length is 179, molecular weight is 20252 Da (predicted); O14966-3: amino acid length is 131, molecular weight is 14964 Da (predicted).
In case of Salmonella enterica serovar Typhimurium (S.typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.typhi-infected cells. -
Subunit
Interacts with LRRK2 (via the N-terminus); this interaction is direct and stimulates kinase activity
-
SwissProt ID
-
Synonyms
RAB7L1, RAB29, Ras-related protein Rab-7L1, Rab-7-like protein 1, Ras-related protein Rab-29
Documentation
[1]. Wang S, et al. A role of Rab29 in the integrity of the trans-Golgi network and retrograde trafficking of mannose-6-phosphate receptor. PLoS One. 2014;9(5):e96242. [Content Brief]
[2]. Purlyte E, et al. Rab29 activation of the Parkinson's disease-associated LRRK2 kinase. EMBO J. 2018 Jan 4;37(1):1-18. [Content Brief]
[3]. Zhu H, et al. Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2. Science. 2023 Dec 22;382(6677):1404-1411. [Content Brief]
[4]. Spanò S, et al. Proteolytic targeting of Rab29 by an effector protein distinguishes the intracellular compartments of human-adapted and broad-host Salmonella. Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18418-23. [Content Brief]
Powered by Bioz
