Perlecan Antibody (YA8898)
(Synonyms: Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2)
Based on 1 Customer Validation
Perlecan Antibody (YA8898) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Perlecan.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IHC-P, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1:100-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
Perlecan Antibody (YA8898) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Perlecan.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 469 kDa
OMIM: 255800
A synthesized peptide derived from human Perlecan.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
Perlecan is an Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. In muscle, it is essential for localizing acetylcholinesterase (AChE) at the neuromuscular junctions (NMJ), most probably acting as an adapter that links the acetylcholinesterase collagenic tail peptide (COLQ) to alpha-dystroglycan, and is therefore involved in the down-regulation of colinergic synaptic transmission (By similarity)[1][2][3].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix, basement membrane; Secreted
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Expression
Tissue_Specificity: Detected in cerebrospinal fluid, fibroblasts and urine (at protein level). -
Isoforms & Post-Translational Modification
Perlecan has an amino acid length of 4391, molecular weight is 468830 Da.
Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide -
Subunit
Has a strong tendency to aggregate in dimers or stellate structures.
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SwissProt ID
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Synonyms
Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2
Documentation
References
[1]. Mongiat M, et al. Endorepellin, a novel inhibitor of angiogenesis derived from the C terminus of perlecan. J Biol Chem. 2003 Feb 7;278(6):4238-49. [Content Brief]
[2]. Gonzalez EM, et al. BMP-1/Tolloid-like metalloproteases process endorepellin, the angiostatic C-terminal fragment of perlecan. J Biol Chem. 2005 Feb 25;280(8):7080-7. [Content Brief]
[3]. Nyström A, et al. Role of tyrosine phosphatase SHP-1 in the mechanism of endorepellin angiostatic activity. Blood. 2009 Nov 26;114(23):4897-906. [Content Brief]
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