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Survivin/BIRC5 Antibody (YA045)

Customer Review

Based on 1 Customer Validation

Survivin/BIRC5 Antibody (YA045) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Survivin/BIRC5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Survivin/BIRC5 Antibody (YA045) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:500 1:50-1:100

Product Details

Description

Survivin/BIRC5 Antibody (YA045) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Survivin/BIRC5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 16 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 16 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within N-terminal human Survivin.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102450

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg) , RAW264.7 (lane 4(20μg),using Survivin Antibody (HY-P80352). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HEK293T (lane 4(20μg), using Survivin/BIRC5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    Flow cytometric analysis of 1X10^6 RAW264.7 cells labeling Survivin Antibody (HY-P80352, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Survivin/BIRC5 is a Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Cytoplasm; Nucleus; Chromosome; Chromosome, centromere; Cytoplasm, cytoskeleton, spindle; Chromosome, centromere, kinetochore; Midbody

  • Expression


    Tissue_specificity:It is expressed only in the fetal kidney and liver, and less frequently in the lung and brain (PubMed: 10626797) . It is highly expressed in adenocarcinomas (lung, pancreatic, colon, breast, and prostate cancers) and high-grade lymphomas (PubMed: 14741722, PubMed: 16329164) . It is also expressed in various renal cell carcinoma lines (PubMed: 10626797) . It is expressed in the cochlea, including the organ of Corti, lateral walls, marginal interdental cells, as well as Schwann cells, cochlear neurons, and spiral ganglion cells (protein level) . It is not expressed in the internal sulcus, external sulcus cells, or Reissner's membrane cells (protein level) (PubMed: 20627126, PubMed: 21364656) .

    Induction:Up-regulated by COMP

  • Subunit

    Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer.

  • SwissProt ID

    O15392

  • Gene ID
    332 [NCBI]
  • Research Field

    Cell Biology

Survivin/BIRC5 Antibody (YA045) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg) , RAW264.7 (lane 4(20μg),using Survivin Antibody (HY-P80352). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HEK293T (lane 4(20μg), using Survivin/BIRC5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 RAW264.7 cells labeling Survivin Antibody (HY-P80352, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Survivin/BIRC5 Antibody (YA045) Product Image
    1/16
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Survivin/BIRC5 Antibody (YA045) Product Image
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg) , RAW264.7 (lane 4(20μg),using Survivin Antibody (HY-P80352). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HEK293T (lane 4(20μg), using Survivin/BIRC5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 RAW264.7 cells labeling Survivin Antibody (HY-P80352, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Experimental Validation Results for Survivin/BIRC5 Antibody (YA045)
  • Survivin/BIRC5 Antibody (YA045) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , Jurkat (lane 3(20μg) , RAW264.7 (lane 4(20μg),using Survivin Antibody (HY-P80352). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Survivin/BIRC5 Antibody (YA045) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HEK293T (lane 4(20μg), using Survivin/BIRC5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Survivin/BIRC5 Antibody (YA045) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Survivin/BIRC5 Antibody (YA045) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using Survivin Antibody (YA045). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80325, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Survivin/BIRC5 Antibody (YA045) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 RAW264.7 cells labeling Survivin Antibody (HY-P80352, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Survivin/BIRC5 Antibody (YA045) Product Image WB
  • Survivin/BIRC5 Antibody (YA045) Product Image WB
  • Survivin/BIRC5 Antibody (YA045) Product Image IHC-P
  • Survivin/BIRC5 Antibody (YA045) Product Image IHC-P
  • Survivin/BIRC5 Antibody (YA045) Product Image FC