Ataxin 3 Antibody (YA2177)
(Synonyms: ATX1; SCA1; D6S504E)Ataxin 3 Antibody (YA2177) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ataxin 3.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IP
-
Reactivity :
Mouse
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IP
IP: Immunoprecipitation
|
|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Ataxin 3 Antibody (YA2177) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ataxin 3.
-
Host Rabbit
-
Clonality Recombinant, Monoclonal
-
Species ReactivityMouse
-
Observed Molecular WeightObserved band size: 105 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 87 kDa
Entrez Gene: 6310 Mouse
SwissProt: P54253 Mouse
Recombinant protein of human Ataxin 1 aa562-693.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Ataxin 3 is a Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development[1].
-
Subcellular Localization
Cytoplasm; Nucleus
-
Expression
Tissue_specificity:Widely distributed throughout the body
Induction:ATXN1 protein levels are directly regulated by PUM1 protein: PUM1 acts by binding to the 3'-UTR of ATXN1 mRNA, affecting ATXN1 mRNA stability and leading to reduced ATXN1 protein levels -
Isoforms & Post-Translational Modification
P54253 has 1 isomers: P54253-1: 86923 Da (predicted).
Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity;Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract;Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract -
Subunit
Homooligomer (PubMed:9097953). Interacts with CIC (By similarity). Interacts with ANP32A, PQBP1, UBQLN4, ATXN1L and USP7 (PubMed:11001934, PubMed:12062018, PubMed:12093161, PubMed:16121196, PubMed:9353121). Directly interacts with RBPJ; this interaction is disrupted in the presence of Notch intracellular domain. Competes with ATXN1L for RBPJ-binding (PubMed:21475249). Found in a complex with CIC and ATXN1L (By similarity)
-
SwissProt ID
-
Synonyms
ATX1; SCA1; D6S504E
-
Research Field
Epigenetics and Nuclear Signaling
Documentation
Powered by Bioz
