Repulsive guidance molecule A Antibody (YA6945)

Cat. No.: HY-P87260: Data Sheet User Guide for Antibodies
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Repulsive guidance molecule A Antibody (YA6945) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Repulsive guidance molecule A.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Repulsive guidance molecule A Antibody (YA6945) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Repulsive guidance molecule A.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 50 kDa;

Observed band size: 37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96B86

Gene ID

56963 [NCBI]

Immunogen

A synthesized peptide derived from human RGMA aa401-435.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Chromatography	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8

Subcellular Localization：Cell membrane

Isoforms & Post-Translational Modification：Q96B86 has three isomers: Q96B86-1: 49361 Da (predicted); Q96B86-3: 47526 Da (predicted); Q96B86-4: 50049 Da (predicted).
Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds

Subunit：Interacts with NEO1, BMP2 and BMP4

Research Field

Neuroscience

Synonyms

Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA; Repulsive guidance molecule A; RGM; RGM domain family member A

Documentation

User Guide for Antibodies (1077 KB)