Repulsive guidance molecule A Antibody (YA6945)
(Synonyms: Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA; Repulsive guidance molecule A; RGM; RGM domain family member A)Repulsive guidance molecule A Antibody (YA6945) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Repulsive guidance molecule A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 |
Product Details
Repulsive guidance molecule A Antibody (YA6945) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Repulsive guidance molecule A.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 37 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
A synthesized peptide derived from human RGMA aa401-435.
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Repulsive guidance molecule A (RGMa) is a GPI-anchored glycoprotein that exhibits repulsive neurite guidance and regulates neuronal differentiation and survival during brain development[1]. Mechanistically, RGMa signals through neogenin and Rho-associated protein kinase, suppressing neurite outgrowth and adult neurogenesis in the dentate gyrus[1][2]. In neural circuit models, RGMa repels entorhinal axons and supports layer-specific termination of the perforant pathway[3]. In disease models, RGMa overexpression induces dopaminergic neuron degeneration and Parkinson’s disease-like behavioral and anatomical changes[4]. Compared with related isoforms, RGMa, RGMb, and RGMc are distinct single-copy genes with discrete expression patterns and unique biological functions[5]. RGM family proteins bind selected BMPs, but RGMa shows lower affinity for most tested BMPs than RGMB or HJV/RGMc[6]. For experimental applications, RGMa blockade increased afferent synapse formation with auditory hair cells, while recombinant RGMa suppressed seizures and mossy fiber sprouting in a PTZ kindling model[7][8].
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Subcellular Localization
Cell membrane
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Isoforms & Post-Translational Modification
Q96B86 has three isomers: Q96B86-1: 49361 Da (predicted); Q96B86-3: 47526 Da (predicted); Q96B86-4: 50049 Da (predicted).
Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds -
Subunit
Interacts with NEO1, BMP2 and BMP4
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SwissProt ID
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Synonyms
Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA; Repulsive guidance molecule A; RGM; RGM domain family member A
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Research Field
Neuroscience
Documentation
References
[1]. Severyn CJ, et al. Molecular biology, genetics and biochemistry of the repulsive guidance molecule family. Biochem J. 2009 Aug 27;422(3):393-403. [Content Brief]
[2]. Isaksen TJ, et al. Repulsive Guidance Molecule A Suppresses Adult Neurogenesis. Stem Cell Reports. 2020 Apr 14;14(4):677-691. [Content Brief]
[3]. Isaksen TJ, et al. Repulsive Guidance Molecule A Regulates Adult Neurogenesis Via the Neogenin Receptor. Neurosci Insights. 2020 Aug 5;15:2633105520948481. [Content Brief]
[4]. Brinks H, et al. The repulsive guidance molecule RGMa is involved in the formation of afferent connections in the dentate gyrus. J Neurosci. 2004 Apr 14;24(15):3862-9. [Content Brief]
[5]. Korecka JA, et al. Repulsive Guidance Molecule a (RGMa) Induces Neuropathological and Behavioral Changes That Closely Resemble Parkinson's Disease. J Neurosci. 2017 Sep 27;37(39):9361-9379. [Content Brief]
[6]. Wu Q, et al. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs). PLoS One. 2012;7(9):e46307. [Content Brief]
[7]. Brugeaud A, et al. Inhibition of repulsive guidance molecule, RGMa, increases afferent synapse formation with auditory hair cells. Dev Neurobiol. 2014 Apr;74(4):457-66. [Content Brief]
[8]. Song M, et al. Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway. Mol Med Rep. 2019 Apr;19(4):3255-3262. [Content Brief]
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