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GSTO1 Antibody (YA2095)

(Synonyms: GSTO1 1; Gsto1; GSTTLp28; GSTX; Gtsttl; p28; SPG-R)
Customer Review

Based on 1 Customer Validation

GSTO1 Antibody (YA2095) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GSTO1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • GSTO1 Antibody (YA2095) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

GSTO1 Antibody (YA2095) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GSTO1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GSTO1 aa40-84.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104459

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), HeLa (lane 2, 20 μg), Jurkat (lane 3, 20 μg), U937 (lane 4, 20 μg), and Rat brain (lane 5, 20 μg) using GSTO1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    Immunohistochemical analysis of paraffin-embedded human fibroma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    GSTO1 is an Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Also has glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Tissue_specificity:Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung

  • Isoforms & Post-Translational Modification

    P78417 has 3 isomers: P78417-1: 27566 Da (predicted); P78417-2: 23709 Da (predicted); P78417-3: 24796 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P78417

  • Gene ID
    9446 [NCBI]
  • Synonyms

    GSTO1 1; Gsto1; GSTTLp28; GSTX; Gtsttl; p28; SPG-R

  • Research Field

    Tags & Cell Markers

GSTO1 Antibody (YA2095) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), HeLa (lane 2, 20 μg), Jurkat (lane 3, 20 μg), U937 (lane 4, 20 μg), and Rat brain (lane 5, 20 μg) using GSTO1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fibroma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image
    1/16
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • GSTO1 Antibody (YA2095) Product Image
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), HeLa (lane 2, 20 μg), Jurkat (lane 3, 20 μg), U937 (lane 4, 20 μg), and Rat brain (lane 5, 20 μg) using GSTO1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fibroma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • Experimental Validation Results for GSTO1 Antibody (YA2095)
  • GSTO1 Antibody (YA2095) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), HeLa (lane 2, 20 μg), Jurkat (lane 3, 20 μg), U937 (lane 4, 20 μg), and Rat brain (lane 5, 20 μg) using GSTO1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GSTO1 Antibody (YA2095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Intrahepatic cholangiocarcinoma‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate cancer‌ tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human fibroma tissue using GSTO1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82350, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • GSTO1 Antibody (YA2095) Product Image WB
  • GSTO1 Antibody (YA2095) Product Image IHC-P
  • GSTO1 Antibody (YA2095) Product Image IHC-P
  • GSTO1 Antibody (YA2095) Product Image IHC-P
  • GSTO1 Antibody (YA2095) Product Image IHC-P
  • GSTO1 Antibody (YA2095) Product Image IHC-P
  • GSTO1 Antibody (YA2095) Product Image IHC-P