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LOX 1 Antibody

(Synonyms: Lectin-type oxidized LDL receptor 1; OLR1; lectin-like low density lipoprotein receptor-1; Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; LOX-1; BLOX1; Contains: Oxidized low-density lipoprotein receptor 1, soluble form A; Oxidized low-density lipoprotein receptor 1, soluble form B; OLR1_HUMAN.)
Customer Review

Based on 1 Customer Validation

LOX 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LOX 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Rat, Rabbit

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • LOX 1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:200 1:50-1:100

Product Details

Description

LOX 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LOX 1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Rat, Rabbit Predicted Reactivity: Mouse,Cow
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

Recombinant protein within Human LOX-1 aa 17-273 / 273.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103253

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LOX 1 Antibody
    Western blot analysis of extracts from MCF-7 (lane 2(20μg) , MCF-7 (lane 3(40μg),using LOX 1 Antibody (HY-P81172). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LOX 1 Antibody
    Immunocytochemistry analysis of HepG2 cells labeling LOX1 with LOX1 Antibody (HY-P81172) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P81172) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LOX 1 Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LOX 1 Antibody
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Unlike native low-density lipoprotein (LDL), modified LDL such as oxidized, carbamylated, or acetylated LDL are not recognized by the native LDL receptor (LDL-R) .1 Rather, modified LDL binds to the lectin-like oxidized LDL receptor-1 (LOX-1) .Upon activation by modified LDL, LOX-1 induces endothelial dysfunction and uptake of the modified lipids.2 Endothelial dysfunction is the first stage in atherogenesis.3 Thus, the identification of LOX-1 through expression cloning as a major receptor for oxLDL in endothelial cells (ECs) supports a putative role for modified LDL in atherogenesis and has taken centre stage in cardiovascular research.

  • Subcellular Localization

    Cell membrane; Lipid-anchor; Cell membrane; Single-pass type II membrane protein; Membrane raft; Secreted

  • Expression


    Induction:Overexpressed in atherosclerotic lesions

    Positive sample: MCF-7, Hela, LOVO, human lung tissue lysate, human kidney tissue, human placenta tissue, mouse brain tissue.

  • Subunit

    Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70 (By similarity)

  • SwissProt ID

    Q9XTA8

  • Gene ID
    4973 [NCBI]
  • Synonyms

    Lectin-type oxidized LDL receptor 1; OLR1; lectin-like low density lipoprotein receptor-1; Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; LOX-1; BLOX1; Contains: Oxidized low-density lipoprotein receptor 1, soluble form A; Oxidized low-density lipoprotein receptor 1, soluble form B; OLR1_HUMAN.

LOX 1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg) , MCF-7 (lane 3(40μg),using LOX 1 Antibody (HY-P81172). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling LOX1 with LOX1 Antibody (HY-P81172) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P81172) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LOX 1 Antibody Product Image
    1/16
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • LOX 1 Antibody Product Image
  • Experimental Validation Results for LOX 1 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg) , MCF-7 (lane 3(40μg),using LOX 1 Antibody (HY-P81172). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LOX 1 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling LOX1 with LOX1 Antibody (HY-P81172) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P81172) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LOX 1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LOX 1 Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • Experimental Validation Results for LOX 1 Antibody
  • LOX 1 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2(20μg) , MCF-7 (lane 3(40μg),using LOX 1 Antibody (HY-P81172). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LOX 1 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling LOX1 with LOX1 Antibody (HY-P81172) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P81172) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LOX 1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LOX 1 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LOX 1 Antibody Product Image WB
  • LOX 1 Antibody Product Image ICC
  • LOX 1 Antibody Product Image IHC-P
  • LOX 1 Antibody Product Image IHC-P