cIAP1 Antibody (YA2500)

Cat. No.: HY-P82755: Fiche technique COA
SDS
User Guide for Antibodies
FAQs
Technical Support

cIAP1 Antibody (YA2500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to cIAP1.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Size	Stock	Quantité
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

or Bulk Inquiry

* Veuillez sélectionner la quantité avant d'ajouter des articles.

cIAP1 Antibody (YA2500) Recommandations en vedette:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description
Références

Description

cIAP1 Antibody (YA2500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to cIAP1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Masse moléculaire

Predicted band size: 70 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13490

Gene ID

329 [NCBI]

Immunogen

A synthetic peptide of human cIAP1 aa29-42.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Pureté	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Hela (lane 3, 15 μg), HT-29 (lane 4, 15 μg), A204 (lane 5, 15 μg), DU145 (lane 6, 15 μg) using cIAP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Present in many human tissues. Mainly expressed in human skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes

Isoforms & Post-Translational Modification：Q13490 has 2 isomers: Q13490-1: 69900 Da (predicted); Q13490-2: 64098 Da (predicted).
Auto-ubiquitinated and degraded by the proteasome in apoptotic cells;Upon stimulation of death receptors, including TNFRSF10B, recruited to receptors and cleaved by caspases. Proteolytic fragments remain associated with the receptors. This cleavage presumably inactivates the protein

Subunit：Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9. Interacts (via BIR domains) with TRAF2; the interaction is required for IKBKE ubiquitination. Interacts with E2F1, RIPK1, RIPK2, RIPK3, RIPK4, BIRC5/survivin and USP19. HSP90AB1 (PubMed:25486457). Interacts with UBXN1 (PubMed:25681446). Interacts with GSK3B (PubMed:18846110). Interacts with several death receptors, inclusing FAS, TNFRSF10A and TNFRSF10B (PubMed:18846110). Recruited to TNFRSF10B in the absence of receptor stimulation. When TNFRSF10B is stimulated, further recruited to the receptor and cleaved by caspases. Proteolytic fragments remain associated with TNFRSF10B (PubMed:18846110)

Database

Entrez Gene: 329 Human

SwissProt: Q13490 Human

OMIM: 601712 Human

Research Field

Cell Biology

Synonyms

cIAP1; C-IAP1; IAP homolog B; Inhibitor of apoptosis protein 2; IAP-2; hIAP-2; hIAP2; API1; IAP2; MIHB; RNF48

Documentation

Select Batch:

Fiche technique (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]

cIAP1 Antibody (YA2500) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Vos produits récemment consultés:

Nom du produit

Requested Quantity *

Nom du demandeur *

Civilité

Adresse e-mail *

Numéro de téléphone *

Department

Nom de l'organisation *

City

État

Country or Region *

Remarques

Nom du produit

Lot #

Nom du demandeur *

Country or Region *

Adresse e-mail *

Remarques

Nom
Email *

	Sorry, but the email address you supplied was invalid.

cIAP1 Antibody (YA2500)

cIAP1 Antibody (YA2500) Recommandations en vedette:

Product Detail

Verification Image

Background

Description

Références

cIAP1 Antibody (YA2500) Related Classifications

Vos produits récemment consultés:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report