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Rad9 Antibody (YA1087)

(Synonyms: RAD9; hRAD9)
Customer Review

Based on 1 Customer Validation

Rad9 Antibody (YA1087) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Rad9.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Rad9 Antibody (YA1087)
  • Rad9 Antibody (YA1087) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Rad9 Antibody (YA1087) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Rad9.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database

Entrez Gene: 5883 Human ;

SwissProt: Q99638 Human ;

Immunogen

Purified recombinant human RAD9A protein fragments expressed in E.coli

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Rad9 Antibody (YA1087)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HT-29 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), COS-7 (lane 4, 15 μg), and Rat testis (lane 5, 15 μg) using RAD9A Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Rad9 component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity[1][2][3][4][5].

  • Subcellular Localization

    Nucleus

  • Subunit

    Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1 (PubMed:10359610, PubMed:10777662, PubMed:10846170, PubMed:10884395, PubMed:15314187, PubMed:15556996, PubMed:15871698, PubMed:15897895, PubMed:16216273, PubMed:17575048, PubMed:20545769, PubMed:31135337). The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2 (PubMed:10884395, PubMed:15556996, PubMed:15871698, PubMed:15897895, PubMed:16216273). The 9-1-1 complex associates with the RAD17-RFC complex (PubMed:12578958). RAD9A interacts with BCL2L1, FEN1, RAD9B, ABL1, RPA1, ATAD5 and RPA2 (PubMed:11971963, PubMed:12628935, PubMed:14500360). Interacts with DNAJC7 (PubMed:11573955). Interacts (when phosphorylated) with TOPBP1 (PubMed:17575048, PubMed:20545769, PubMed:31135337)

  • SwissProt ID

    Q99638

  • Gene ID
    5883 [NCBI]
  • Synonyms

    RAD9; hRAD9

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Bessho T, et al. Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease. J Biol Chem. 2000 Mar 17;275(11):7451-4. [Content Brief]

[2]. Delacroix S, et al. The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1. Genes Dev. 2007 Jun 15;21(12):1472-7. [Content Brief]

[3]. Takeishi Y, et al. Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1. Genes Cells. 2010 Jun;15(7):761-71. [Content Brief]

[4]. Cotta-Ramusino C, et al. A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. Science. 2011 Jun 10;332(6035):1313-7. [Content Brief]

[5]. Bigot N, et al. Phosphorylation-mediated interactions with TOPBP1 couple 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint. Elife. 2019 May 28;8:. [Content Brief]

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Rad9 Antibody (YA1087) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HT-29 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), COS-7 (lane 4, 15 μg), and Rat testis (lane 5, 15 μg) using RAD9A Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Rad9 Antibody (YA1087) Product Image
    1/16
  • Experimental Validation Results for Rad9 Antibody (YA1087)
  • Rad9 Antibody (YA1087) Product Image
  • Experimental Validation Results for Rad9 Antibody (YA1087)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HT-29 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), COS-7 (lane 4, 15 μg), and Rat testis (lane 5, 15 μg) using RAD9A Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Rad9 Antibody (YA1087)
  • Rad9 Antibody (YA1087) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HT-29 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), COS-7 (lane 4, 15 μg), and Rat testis (lane 5, 15 μg) using RAD9A Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Rad9 Antibody (YA1087) Product Image WB