Rab25 Antibody (YA5035)
(Synonyms: RAB25; CATX8; Ras-related protein Rab-25; CATX-8)Rab25 Antibody (YA5035) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Rab25.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-2000 |
Product Details
Rab25 Antibody (YA5035) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Rab25.
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Host Rabbit
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Clonality Monoclonal, Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 23 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 23 kDa
A synthesized peptide derived from human Rab25. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Rab25 is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB25 regulates epithelial cell differentiation, proliferation and survival, thereby playing key roles in tumorigenesis. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis[1].
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Subcellular Localization
Cell membrane; Lipid-anchor; Cytoplasmic side; Cytoplasmic vesicle; Cell projection, pseudopodium membrane
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Expression
Tissue_specificity:This gene is expressed only in epithelial cells (PubMed: 15502842) . It is expressed in both ovarian epithelial (NOE) and breast tissue. It is also expressed in ovarian cancer; its expression level is higher than in NOE cells. Expression in ovarian cancer is stage-dependent, with higher expression levels in stage III and IV ovarian cancer than in early-stage ovarian cancer. It is also expressed in breast cancer; its expression level is higher than in normal breast tissue. -
Subunit
Interacts (GTP-bound form) with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4 (PubMed:11495908, PubMed:26032412). Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent (PubMed:17925226). Interacts with ITGAV (PubMed:17925226). Associates with the integrin alpha-V/beta-1 heterodimer. Interacts with VPS33B (PubMed:28017832)
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SwissProt ID
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Synonyms
RAB25; CATX8; Ras-related protein Rab-25; CATX-8
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