Rab25 Antibody (YA5035)

Art. -Nr.: HY-P85343: Data Sheet User Guide for Antibodies
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Rab25 Antibody (YA5035) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Rab25.

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

Rab25 Antibody (YA5035) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Rab25.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molekulargewicht

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P57735

Gene ID

57111 [NCBI]

Immunogen

A synthesized peptide derived from human Rab25. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Reinheit	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB25 regulates epithelial cell differentiation, proliferation and survival, thereby playing key roles in tumorigenesis (PubMed:17925226). Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway (By similarity). Together with MYO5B regulates transcytosis (By similarity)

Subcellular Localization：Cell membrane; Lipid-anchor; Cytoplasmic side; Cytoplasmic vesicle; Cell projection, pseudopodium membrane

Expression：
Tissue_specificity:This gene is expressed only in epithelial cells (PubMed: 15502842) . It is expressed in both ovarian epithelial (NOE) and breast tissue. It is also expressed in ovarian cancer; its expression level is higher than in NOE cells. Expression in ovarian cancer is stage-dependent, with higher expression levels in stage III and IV ovarian cancer than in early-stage ovarian cancer. It is also expressed in breast cancer; its expression level is higher than in normal breast tissue.

Subunit：Interacts (GTP-bound form) with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4 (PubMed:11495908, PubMed:26032412). Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent (PubMed:17925226). Interacts with ITGAV (PubMed:17925226). Associates with the integrin alpha-V/beta-1 heterodimer. Interacts with VPS33B (PubMed:28017832)

Synonyms

RAB25; CATX8; Ras-related protein Rab-25; CATX-8

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/mda-mb-231-cell.html [Content Brief]