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CD209 Antibody (YA4510)

(Synonyms: CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1)
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Based on 1 Customer Validation

CD209 Antibody (YA4510) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD209.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD209 Antibody (YA4510)
  • CD209 Antibody (YA4510) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

CD209 Antibody (YA4510) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD209.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Immunogen

Purified recombinant fragment of human CD209 (AA: extra 270-404) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD209 Antibody (YA4510)
    Flow cytometric analysis of 1X10^6 Ramos cells labeling CD209 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    CD209 is a Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response; On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells; (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2; (Microbial infection) Acts as an attachment receptor for Ebolavirus; (Microbial infection) Acts as an attachment receptor for Cytomegalovirus; (Microbial infection) Acts as an attachment receptor for HCV; (Microbial infection) Acts as an attachment receptor for Dengue virus; (Microbial infection) Acts as an attachment receptor for Measles virus; (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1; (Microbial infection) Acts as an attachment receptor for Influenzavirus A; (Microbial infection) Acts as an attachment receptor for SARS-CoV; (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus; (Microbial infection) Acts as an attachment receptor for Lassa virus. Acts as an attachment receptor for Marburg virusn; (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus; (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus; (Microbial infection) Acts as an attachment receptor for West-nile virus; (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted

  • Expression


    Tissue_specificity:It is mainly expressed in dendritic cells and the tissues in which dendritic cells reside. It is also found in placental macrophages, placental vascular endothelial cells, peripheral blood monocytes, and THP-1 monocytes.

  • Isoforms & Post-Translational Modification

    Q9NNX6 has 12 isomers: Q9NNX6-1: 45775 Da (predicted); Q9NNX6-2: 45031 Da (predicted); Q9NNX6-3: 35236 Da (predicted); Q9NNX6-4: 18646 Da (predicted); Q9NNX6-5: 45571 Da (predicted); Q9NNX6-6: 43330 Da (predicted); Q9NNX6-7: 41009 Da (predicted); Q9NNX6-8: 30427 Da (predicted); Q9NNX6-9: 4046 Da (predicted); Q9NNX6-10: 43126 Da (predicted); Q9NNX6-11: 37844 Da (predicted); Q9NNX6-12: 33874 Da (predicted).

  • Subunit

    Homotetramer. Interacts with C1QBP; the interaction is indicative for a C1q:C1QBP:CD209 signaling complex. Interacts with ICAM2 and ICAM3 by binding to mannose-like carbohydrates. Interacts (via C-type lectin domain) with CEACAM1 (via Lewis X moieties); this interaction is regulated by the glycosylation pattern of CEACAM1 on cell types and regulates contact between dendritic cells and neutrophils (PubMed:16246332)

  • SwissProt ID

    Q9NNX6

  • Gene ID
    30835 [NCBI]
  • Synonyms

    CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

CD209 Antibody (YA4510) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling CD209 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • CD209 Antibody (YA4510) Product Image
    1/16
  • Experimental Validation Results for CD209 Antibody (YA4510)
  • CD209 Antibody (YA4510) Product Image
  • Experimental Validation Results for CD209 Antibody (YA4510)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling CD209 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for CD209 Antibody (YA4510)
  • CD209 Antibody (YA4510) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Ramos cells labeling CD209 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • CD209 Antibody (YA4510) Product Image FC