2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Cytokeratin 1 Antibody (YA2665)

Cytokeratin 1 Antibody (YA2665)

Art. -Nr.: HY-P82920
COA
SDS
User Guide for Antibodies Technical Support

Cytokeratin 1 Antibody (YA2665) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

  • Verweise

Beschreibung

Cytokeratin 1 Antibody (YA2665) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 66 kDa;
Observed band size: 66 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Cytokeratin 1 aa150-300.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Cytokeratin 1 (HY-P82920) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK
Subcellular Localization：Cell membrane; Cytoplasm
Expression：
Tissue_specificity:This protein originates from the foreskin of newborns. The 67 kDa type II keratin is expressed in the terminally differentiated epidermis.

Induction:Repressed in keratinocytes by all-trans retinoic acid (ATRA) , via reduction of mRNA stability
Subunit：Heterotetramer of two type I and two type II keratins (PubMed:24940650, PubMed:27595935). Heterodimer with KRT10 (PubMed:24940650, PubMed:27595935). Two heterodimers of KRT1 and KRT10 form a heterotetramer (PubMed:27595935). Forms a heterodimer with KRT14; the interaction is more abundant in the absence of KRT5 (By similarity). Interacts with PLEC isoform 1C, when in a heterodimer with KRT10 (PubMed:24940650). Interacts with ITGB1 in the presence of RACK1 and SRC, and with RACK1 (PubMed:17956333). Interacts with C1QBP; the association represents a cell surface kininogen receptor (PubMed:21544310). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132)
RRID
Database

Entrez Gene: 3848 Human ; 16678 Mouse ; 300250 Rat

SwissProt: P04264 Human ; P04104 Mouse ; Q6IMF3 Rat

OMIM: 113800 Human

Research Field

Signal Transduction
Synonyms
67 kDa cytokeratin; CK1; EHK; Epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; Keratin; KRT 1; KRT1A; NEPPK; type II cytoskeletal 1; Type-II keratin Kb1
Dokumentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
Verweise

Cytokeratin 1 Antibody (YA2665) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cytokeratin 1 Antibody (YA2665)
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