PU.1/SPI1 Antibody (YA1116)(PBS only)

Cat. No.: HY-P81371A: Fiche technique User Guide for Antibodies
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PU.1/SPI1 Antibody (YA1116) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PU.1/SPI1.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

* Veuillez sélectionner la quantité avant d'ajouter des articles.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

PU.1/SPI1 Antibody (YA1116) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PU.1/SPI1.

Host

Rabbit

Masse moléculaire

Predicted band size: 31 kDa;

Observed band size: 31 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Hamster

SwissProt ID

P17433

Gene ID

6688 [NCBI]

Immunogen

A synthetic peptide of mouse PU.1/Spi1.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Pureté	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites (PubMed:8079170). Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (By similarity). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:8079170). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:2180582). In vitro can bind RNA and interfere with pre-mRNA splicing (PubMed:8626664)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed in spleen, thymus and bone-marrow macrophages

Subunit：Binds DNA as a monomer. Directly interacts with CEBPD/NF-IL6-beta; this interaction does not affect DNA-binding properties of each partner (PubMed:7594592).

Research Field

Immunology

Synonyms

SPI1; Transcription factor PU.1; 31 kDa-transforming protein

Documentation

User Guide for Antibodies (1077 KB)