RhoGDI Antibody (YA4080)(PBS only)

Cat. No.: HY-P84383A: Data Sheet User Guide for Antibodies
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RhoGDI Antibody (YA4080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RhoGDI.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RhoGDI Antibody (YA4080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RhoGDI.

Host

Mouse

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

P52565

Gene ID

396 [NCBI]

Immunogen

Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1

Subcellular Localization：Cytoplasm

Isoforms & Post-Translational Modification：P52565 has 2 isomers: P52565-1: 23207 Da (predicted); P52565-2: 18233 Da (predicted).

Subunit：Interacts with FER (PubMed:21122136). Forms a heterodimer with RAC1 (PubMed:23434736).

Synonyms

GDIA1; RHOGDI; RHOGDI-1

Documentation

User Guide for Antibodies (1077 KB)