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NR0B1/DAX1 Antibody (YA2341)

(Synonyms: AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2)
Customer Review

Based on 1 Customer Validation

NR0B1/DAX1 Antibody (YA2341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NR0B1/DAX1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • NR0B1/DAX1 Antibody (YA2341) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

NR0B1/DAX1 Antibody (YA2341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NR0B1/DAX1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Species Reactivity Database

Entrez Gene: 190 Human

SwissProt: P51843 Human

Immunogen

A synthesized peptide derived from human NR0B1 / Dax1 aa1-50/470.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104697

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
    Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using NR0B1/DAX1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
    Immunocytochemistry analysis of SH-SY5Y cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
    Immunocytochemistry analysis of A549 cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    NR0B1/DAX1 is a Nuclear receptor that lacks a DNA-binding domain and acts as a corepressor that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency[1][2][3][4].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Isoforms & Post-Translational Modification

    P51843 has 2 isomers: P51843-1: 51718 Da (predicted); P51843-2: 43590 Da (predicted).

  • Subunit

    Homodimer (PubMed:16709599). Interacts with NR5A1, NR5A2, NR0B2 and with COPS2 (PubMed:10713076, PubMed:12482977, PubMed:16709599, PubMed:26416531, PubMed:32433991). Interacts with ESRRB; represses ESRRB activity at the GATA6 promoter (By similarity)

  • SwissProt ID

    P51843

  • Gene ID
    190 [NCBI]
  • Synonyms

    AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2

  • Research Field

    Neuroscience

NR0B1/DAX1 Antibody (YA2341) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using NR0B1/DAX1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NR0B1/DAX1 Antibody (YA2341) Product Image
    1/16
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • NR0B1/DAX1 Antibody (YA2341) Product Image
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using NR0B1/DAX1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • Experimental Validation Results for NR0B1/DAX1 Antibody (YA2341)
  • NR0B1/DAX1 Antibody (YA2341) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using NR0B1/DAX1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • NR0B1/DAX1 Antibody (YA2341) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NR0B1/DAX1 Antibody (YA2341) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling NR0B1/DAX1 with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with NR0B1/DAX1 Antibody (YA2341) (HY-P82596) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NR0B1/DAX1 Antibody (YA2341) Product Image WB
  • NR0B1/DAX1 Antibody (YA2341) Product Image ICC
  • NR0B1/DAX1 Antibody (YA2341) Product Image ICC