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FGFBP1 Antibody (YA9962)

(Synonyms: FGF-BP; FGF-BP1; FGFBP; FGFBP-1; HBP17)

FGFBP1 Antibody (YA9962) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FGFBP1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FGFBP1 Antibody (YA9962)
  • FGFBP1 Antibody (YA9962) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-2000

Product Details

Description

FGFBP1 Antibody (YA9962) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FGFBP1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 26.1kDa
Immunogen

Human recombinant protein fragment corresponding to amino acids 24-234 of human FGFBP1 produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FGFBP1 Antibody (YA9962)
    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HepG2 (lane 3), HeLa (lane 4), and DU145 (lane 5) using FGFBP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    FGFBP1 acts as a carrier protein that release fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth

  • Subcellular Localization

    Secreted, extracellular space; Cell membrane

  • Expression


    Tissue_Specificity: Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo-epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas.
    Induction: Up-regulated in epithelial cells after skin injury. Keratinocyte mitogens.

  • Isoforms & Post-Translational Modification

    FGFBP1 has an amino acid length of 234, molecular weight is 26264 Da.

  • Subunit

    Found in a complex with FGFBP1, FGF1 and FGF2.

  • SwissProt ID

    Q14512

  • Synonyms

    FGF-BP; FGF-BP1; FGFBP; FGFBP-1; HBP17

FGFBP1 Antibody (YA9962) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HepG2 (lane 3), HeLa (lane 4), and DU145 (lane 5) using FGFBP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FGFBP1 Antibody (YA9962) Product Image
    1/16
  • Experimental Validation Results for FGFBP1 Antibody (YA9962)
  • FGFBP1 Antibody (YA9962) Product Image
  • Experimental Validation Results for FGFBP1 Antibody (YA9962)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HepG2 (lane 3), HeLa (lane 4), and DU145 (lane 5) using FGFBP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for FGFBP1 Antibody (YA9962)
  • FGFBP1 Antibody (YA9962) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HepG2 (lane 3), HeLa (lane 4), and DU145 (lane 5) using FGFBP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FGFBP1 Antibody (YA9962) Product Image WB