Rab5a Antibody (YA3869)(PBS only)

Cat. No.: HY-P84172A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Rab5a Antibody (YA3869) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Rab5a.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Rab5a Antibody (YA3869) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Rab5a.

Host

Mouse

Molecular Weight

Predicted band size: 24 kDa;

Observed band size: 24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P20339

Gene ID

5868 [NCBI]

Immunogen

Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity)

Subcellular Localization：Cell membrane; Lipid-anchor; Cytoplasmic side; Early endosome membrane; Lipid-anchor; Melanosome; Cytoplasmic vesicle; Cell projection, ruffle; Membrane; Cytoplasm, cytosol; Cytoplasmic vesicle, phagosome membrane; Endosome membrane

Isoforms & Post-Translational Modification：P20339 has 2 isomers: P20339-1: 23659 Da (predicted); P20339-2: 22178 Da (predicted).
Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2;(Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3

Subunit：Interacts with GDI1; this promotes dissociation from membranes; phosphorylation at Ser-84 disrupts this interaction (PubMed:23815289, PubMed:29125462).

Synonyms

RAB5

Documentation

User Guide for Antibodies (1077 KB)