PP1C gamma Antibody (YA5210)

Cat. No.: HY-P85518: Data Sheet User Guide for Antibodies
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PP1C gamma Antibody (YA5210) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PP1C gamma.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PP1C gamma Antibody (YA5210) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PP1C gamma.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 38 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P36873

Gene ID

5501 [NCBI]

Immunogen

Purified recombinant human PP1C protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
FC FC: Flow Cytometry	1:50-100

Sensitivity	Endogenous	Purity	affinity chromatography.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in Ascitic fluid containing 0.03% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Protein phosphatase that associates witH2O2er 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets (PubMed:17936702, PubMed:25012651). Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1 (PubMed:17936702). Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of cH2O2atin structure and cell cycle progression during the transition from mitosis into interphase (PubMed:20516061). In balance with CSNK1D and CSNK1E, determines the circadian period length, tH2O2gh the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed:21712997). May dephosphorylate CSNK1D and CSNK1E (By similarity). Regulates the recruitment of the SKA complex to kinetochores (PubMed:28982702). Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Together with PPP1CA (PP1-alpha subunit), dephosphorylates IFIH1/MDA5 and RIG-I leading to their activation and a functional innate immune response (PubMed:23499489). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed:35768504, PubMed:35831509). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:35768504, PubMed:35831509). Dephosphorylates MKI67 at the onset of anaphase (PubMed:25012651). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed:35768504, PubMed:35831509)

Subcellular Localization：Cytoplasm; Nucleus; Nucleus, nucleolus; Nucleus, nucleoplasm; Nucleus speckle; CH2O2osome, centromere, kinetochore; Cleavage furrow; Midbody; Mitochondrion; Cytoplasm, cytoskeleton, microtubule organizing center

Expression：
Induction:Up-regulated in synovial fluid mononuclear cells and peripheral blood mononuclear cells from with rheumatoid arthritis

Isoforms & Post-Translational Modification：P36873 has 2 isomers: P36873-1: 36984 Da (predicted); P36873-2: 38518 Da (predicted).
Phosphorylated by NEK2

Subunit：PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits.

Synonyms

EC 3.1.3.16; PP 1G; PP-1G; PP1G; PP1G_HUMAN; PP1gamma; PPP 1G; PPP1CC; PPP1CC protein; PPP1G; Protein phosphatase 1 catalytic subunit gamma isoform; Protein phosphatase 1C catalytic subunit; Protein phosphatase 1C subunit; Protein phosphatase 2C gamma isoform; Serine/threonine phosphatase 1 gamma; Serine/threonine protein phosphatase PP1 gamma catalytic subunit; Serine/threonine-protein phosphatase PP1-gamma catalytic subunit.

Documentation

User Guide for Antibodies (1077 KB)