HtrA2/Omi Antibody (YA5287)

Cat. No.: HY-P85595: Data Sheet User Guide for Antibodies
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HtrA2/Omi Antibody (YA5287) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to HtrA2/Omi.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HtrA2/Omi Antibody (YA5287) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to HtrA2/Omi.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 49 kDa;

Species Reactivity

Human

SwissProt ID

O43464

Gene ID

27429 [NCBI]

Immunogen

Recombinant human HtrA2/Omi protein.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed:10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism (PubMed:15200957). Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6 (PubMed:36758104, PubMed:36758105). Cleaves THAP5 and promotes its degradation during apoptosis (PubMed:19502560); Seems to be proteolytically inactive

Subcellular Localization：Mitochondrion intermembrane space; Mitochondrion membrane; Single-pass membrane protein; Endoplasmic reticulum

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：O43464 has 4 isomers: O43464-1: 48841 Da (predicted); O43464-2: 38493 Da (predicted); O43464-3: 46382 Da (predicted); O43464-4: 39914 Da (predicted).
Ubiquitinated by BIRC6; this activity is inhibited by DIABLO/SMAC;Autoproteolytically activated

Subunit：Homotrimer (PubMed:36758104, PubMed:36758105). Interacts with MXI2. Interacts with THAP5 under apoptotic conditions. The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with AREL1 (via HECT domain); in the cytoplasm following induction of apoptosis (PubMed:23479728)

Synonyms

High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA, E. coli, homolog of, 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serin

Documentation

User Guide for Antibodies (1077 KB)