RPL11 Antibody
(Synonyms: Large ribosomal subunit protein uL5, 60S ribosomal protein L11, CLL-associated antigen KW-12, RPL11)RPL11 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RPL11.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
RPL11 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RPL11.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 20 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 20 kDa
Synthetic peptide corresponding to the C-term region of human RPL11.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RPL11 is a Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Promotes nucleolar location of PML (By similarity)[1][2][3][4][5].
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Subcellular Localization
Nucleus, nucleolus; Cytoplasm
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Isoforms & Post-Translational Modification
RPL11 has 2 isoforms, P62913-1: amino acid length is 178, molecular weight is 20252 Da (predicted); P62913-2: amino acid length is 177, molecular weight is 20124 Da (predicted).
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Subunit
Component of the large ribosomal subunit (LSU).
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SwissProt ID
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Synonyms
Large ribosomal subunit protein uL5, 60S ribosomal protein L11, CLL-associated antigen KW-12, RPL11
Documentation
[1]. Cmejla R, et al. Identification of mutations in the ribosomal protein L5 (RPL5) and ribosomal protein L11 (RPL11) genes in Czech patients with Diamond-Blackfan anemia. Hum Mutat. 2009 Mar;30(3):321-7. [Content Brief]
[2]. Liang X, et al. Structural snapshots of human pre-60S ribosomal particles before and after nuclear export. Nat Commun. 2020 Jul 15;11(1):3542. [Content Brief]
[3]. Odintsova TI, et al. Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing. J Protein Chem. 2003 Apr;22(3):249-58. [Content Brief]
[4]. Gazda HT, et al. Ribosomal protein L5 and L11 mutations are associated with cleft palate and abnormal thumbs in Diamond-Blackfan anemia patients. Am J Hum Genet. 2008 Dec;83(6):769-80. [Content Brief]
[5]. Sloan KE, et al. The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress. Cell Rep. 2013 Oct 17;5(1):237-47. [Content Brief]
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