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TTF-1 Antibody (YA2297)(PBS only)

(Synonyms: NKX2-1; NKX2A; TITF1; TTF1; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1)

TTF-1 Antibody (YA2297) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TTF-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Hamster

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TTF-1 Antibody (YA2297)(PBS only)
  • TTF-1 Antibody (YA2297)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

TTF-1 Antibody (YA2297) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TTF-1.

  • Host Rabbit
  • Species Reactivity
    Human, Hamster
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Immunogen

A synthetic peptide of human TTF1

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TTF-1 Antibody (YA2297)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), DU145 (lane 3, 15 μg), COLO320 (lane 4, 15 μg), Rat lung (lane 5, 15 μg), Mouse lung (lane 6, 15 μg) using Thyroid Transcription Factor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    TTF-1 transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:thyroid and lungs

  • Isoforms & Post-Translational Modification

    P43699 has 2 isomers: P43699-1: 38596 Da (predicted); P43699-3: 41737 Da (predicted).
    Phosphorylated on serine residues by STK3/MST2

  • Subunit

    Interacts with WWTR1

  • SwissProt ID

    P43699

  • Gene ID
    7080 [NCBI]
  • Synonyms

    NKX2-1; NKX2A; TITF1; TTF1; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1

  • Research Field

    Epigenetics and Nuclear Signaling

TTF-1 Antibody (YA2297)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), DU145 (lane 3, 15 μg), COLO320 (lane 4, 15 μg), Rat lung (lane 5, 15 μg), Mouse lung (lane 6, 15 μg) using Thyroid Transcription Factor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TTF-1 Antibody (YA2297)(PBS only) Product Image
    1/16
  • Experimental Validation Results for TTF-1 Antibody (YA2297)(PBS only)
  • TTF-1 Antibody (YA2297)(PBS only) Product Image
  • Experimental Validation Results for TTF-1 Antibody (YA2297)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), DU145 (lane 3, 15 μg), COLO320 (lane 4, 15 μg), Rat lung (lane 5, 15 μg), Mouse lung (lane 6, 15 μg) using Thyroid Transcription Factor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TTF-1 Antibody (YA2297)(PBS only)
  • TTF-1 Antibody (YA2297)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), DU145 (lane 3, 15 μg), COLO320 (lane 4, 15 μg), Rat lung (lane 5, 15 μg), Mouse lung (lane 6, 15 μg) using Thyroid Transcription Factor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TTF-1 Antibody (YA2297)(PBS only) Product Image WB