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Cat. No.: HY-P85970

p27 KIP 1 Antibody (YA5662)

Name: p27 KIP 1 Antibody (YA5662)
Brand: MedChemExpress
SKU: HY-P85970
Availability: PreOrder

p27 KIP 1 Antibody (YA5662) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to p27 KIP 1.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	ELISA ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio	1:200-1000	1:500-2000	1:100-500	1:1000-5000

Description

p27 KIP 1 Antibody (YA5662) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to p27 KIP 1.

Host Mouse
Clonality Monoclonal
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 22 kDa

Immunogen

Synthesized peptide derived from human p27Kip1 AA range: 100-198

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Appearance

Liquid
Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

p27 KIP 1 is important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry^[1].
Subcellular Localization

Nucleus; Cytoplasm; Endosome
Expression

Tissue_specificity:It is expressed in the kidney (protein level) (PubMed: 15509543) . It is expressed in all tissues tested (PubMed: 8033212) . The highest content is found in skeletal muscle, and the lowest content is found in the liver and kidney (PubMed: 8033212) .

Induction:Maximal levels in quiescence cells and early G (1) . Levels decrease after mitogen stimulation as cells progress toward S-phase
Subunit

Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.
SwissProt ID

P46527
Gene ID

1027 [NCBI]