ASF1B Antibody (YA4527)(PBS only)
(Synonyms: CIA-II)ASF1B Antibody (YA4527) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASF1B.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, FC, ELISA
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Reactivity :
Human, Mouse, Monkey
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
ASF1B Antibody (YA4527) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASF1B.
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Host Mouse
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Species ReactivityHuman, Mouse, Monkey
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 22 kDa
Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ASF1B is a Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for gonad development[1][2][3][4][5][6][7][8].
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Subcellular Localization
Nucleus; Cytoplasm, cytosol
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Expression
Tissue_specificity:Highly expressed in testis and at lower levels in colon, small intestine and thymus -
Subunit
Interacts with histone H3 (via C-terminus), including histone H3.1, H3.2 and H3.3, and histone H4; the interaction with H3 is direct (PubMed:12842904, PubMed:14718166, PubMed:15664198, PubMed:16537536, PubMed:22195965). Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex (PubMed:11897662, PubMed:14718166, PubMed:16537536, PubMed:16980972). Interacts with HAT1, NASP and TAF1 (PubMed:12842904). Found in a soluble complex with NASP and histones H3 and H4; the interaction with NASP is probably indirect and mediated by H3-H4 (PubMed:22195965). Interacts with CDAN1 (PubMed:22407294). Found in a cytosolic complex with IPO4 and histones H3 and H4 (PubMed:20953179, PubMed:21454524, PubMed:22407294). Interacts with CREBBP (PubMed:24616510)
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SwissProt ID
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Synonyms
CIA-II
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