ASF1B Antibody (YA4527)(PBS only)

Cat. No.: HY-P84830A: Data Sheet User Guide for Antibodies
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ASF1B Antibody (YA4527) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASF1B.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ASF1B Antibody (YA4527) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASF1B.

Host

Mouse

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

Q9NVP2

Gene ID

55723 [NCBI]

Immunogen

Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251, PubMed:21454524, PubMed:26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed:20953179, PubMed:21454524, PubMed:26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Required for gonad development (PubMed:12842904)

Subcellular Localization：Nucleus; Cytoplasm, cytosol

Expression：
Tissue_specificity:Highly expressed in testis and at lower levels in colon, small intestine and thymus

Subunit：Interacts with histone H3 (via C-terminus), including histone H3.1, H3.2 and H3.3, and histone H4; the interaction with H3 is direct (PubMed:12842904, PubMed:14718166, PubMed:15664198, PubMed:16537536, PubMed:22195965). Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex (PubMed:11897662, PubMed:14718166, PubMed:16537536, PubMed:16980972). Interacts with HAT1, NASP and TAF1 (PubMed:12842904). Found in a soluble complex with NASP and histones H3 and H4; the interaction with NASP is probably indirect and mediated by H3-H4 (PubMed:22195965). Interacts with CDAN1 (PubMed:22407294). Found in a cytosolic complex with IPO4 and histones H3 and H4 (PubMed:20953179, PubMed:21454524, PubMed:22407294). Interacts with CREBBP (PubMed:24616510)

Synonyms

CIA-II

Documentation

User Guide for Antibodies (1077 KB)