Copy Link Print Information

RPS6 Antibody (YA5908)(PBS only)

(Synonyms: RPS6; OK/SW-cl.2; 40S ribosomal protein S6; Phosphoprotein NP33)
Customer Review

Based on 1 Customer Validation

RPS6 Antibody (YA5908) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RPS6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RPS6 Antibody (YA5908)(PBS only)
  • RPS6 Antibody (YA5908)(PBS only) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:200-1:500 1:200-1:1000 1:200-1:500

Product Details

Description

RPS6 Antibody (YA5908) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RPS6.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Immunogen

Synthetic peptide derived from human S6 Ribosomal Protein.

Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719107

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RPS6 Antibody (YA5908)(PBS only)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), 293 (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using RPS6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    RPS6 component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Nucleus, nucleolus

  • Subunit

    Component of the small ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3 (PubMed:34516797)

  • SwissProt ID

    P62753

  • Gene ID
    6194 [NCBI]
  • Synonyms

    RPS6; OK/SW-cl.2; 40S ribosomal protein S6; Phosphoprotein NP33

RPS6 Antibody (YA5908)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), 293 (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using RPS6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RPS6 Antibody (YA5908)(PBS only) Product Image
    1/16
  • Experimental Validation Results for RPS6 Antibody (YA5908)(PBS only)
  • RPS6 Antibody (YA5908)(PBS only) Product Image
  • Experimental Validation Results for RPS6 Antibody (YA5908)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), 293 (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using RPS6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RPS6 Antibody (YA5908)(PBS only)
  • RPS6 Antibody (YA5908)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), 293 (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using RPS6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RPS6 Antibody (YA5908)(PBS only) Product Image WB