Copy Link Print Information

ZO-1/TJP1 Antibody (YA5942)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

ZO-1/TJP1 Antibody (YA5942) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZO-1/TJP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat, Pig

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA5942)
  • ZO-1/TJP1 Antibody (YA5942) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) ZO-1/TJP1 Antibody (YA5942)

More

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:4000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

ZO-1/TJP1 Antibody (YA5942) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZO-1/TJP1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Pig
  • Observed Molecular Weight
    Observed band size: 220 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 195 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA5942)
    Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), MCF-7 (lane 3), 293T (lane 4), SH-SY5Y (lane 5), A549 (lane 6), and K562 (lane 7) using ZO-1/TJP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    ZO-1/TJP1 is a TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction, tight junction; Cell junction; Cell junction, gap junction; Cell projection, podosome

  • Expression


    Tissue_specificity:Isoforms containing the α subunit are present at most epithelial cell junctions. Short isoforms are present at highly specialized epithelial junctions, such as those of endothelial cells, glomeruli, and seminiferous tubules.

  • Isoforms & Post-Translational Modification

    Q07157 has 2 isomers: Q07157-1: 195459 Da (predicted); Q07157-2: 186966 Da (predicted).
    Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF) (PubMed:19332538, PubMed:7542259). This response is dependent on an intact actin microfilament system (PubMed:7542259). Dephosphorylated by PTPRJ (PubMed:19332538)

  • Subunit

    Homodimer (PubMed:16737969, PubMed:17928286).

  • SwissProt ID

    Q07157

  • Gene ID
    7082 [NCBI]

ZO-1/TJP1 Antibody (YA5942) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), MCF-7 (lane 3), 293T (lane 4), SH-SY5Y (lane 5), A549 (lane 6), and K562 (lane 7) using ZO-1/TJP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ZO-1/TJP1 Antibody (YA5942) Product Image
    1/16
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA5942)
  • ZO-1/TJP1 Antibody (YA5942) Product Image
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA5942)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), MCF-7 (lane 3), 293T (lane 4), SH-SY5Y (lane 5), A549 (lane 6), and K562 (lane 7) using ZO-1/TJP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA5942)
  • ZO-1/TJP1 Antibody (YA5942) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), MCF-7 (lane 3), 293T (lane 4), SH-SY5Y (lane 5), A549 (lane 6), and K562 (lane 7) using ZO-1/TJP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ZO-1/TJP1 Antibody (YA5942) Product Image WB