LRP2 Antibody (YA6390)(PBS only)
(Synonyms: LRP2_HUMAN; Low-density lipoprotein receptor-related protein 2; LDL receptor related protein 2; DBS; GP330; LRP-2; Glycoprotein 330(gp330); Megalin; )LRP2 Antibody (YA6390) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LRP2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
IHC-P, WB, IF-Tissue, IHC-F, ICC/IF
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Reactivity :
Mouse, Rat, Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|---|---|
| Dilution Ratio | 1:100-500 | 1:500-2000 | 1:100-500 | 1:100-500 | 1:50-200 |
Product Details
LRP2 Antibody (YA6390) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LRP2.
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Host Rabbit
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Species ReactivityMouse, Rat, Human
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Calculated Molecular Weight Predicted band size: 519 kDa
Recombinant mouse LRP2 protein aa 4551-4660/4660
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Low-density lipoprotein receptor-related protein 2 (LRP2), also known as megalin, is an endocytic receptor expressed on the apical surface of epithelial cells that internalizes diverse ligands, including nutrients, hormones, morphogens, and extracellular matrix proteins[1]. Mechanistically, LRP2 modulates sonic hedgehog (SHH) signaling in stem and progenitor cell niches, playing crucial roles in brain, eye, and heart development[2]. This receptor participates in both lysosomal degradation and transcytosis, with its surface availability regulated by phosphorylation, extracellular domain shedding, intramembrane proteolysis, and exosome secretion[1]. LRP2 dysfunction has been linked to congenital syndromes, diabetic nephropathy, Dent disease, Lowe syndrome, Alzheimer's disease, and neural regeneration deficits[1][2]. Compared with related LDL receptor family members, LRP2 uniquely acts as an auxiliary receptor for SHH, whereas LRP5/6 primarily function as Wnt co-receptors and LRP1 modulates inflammatory signaling[3][2][4]. In experimental models, LRP2 expression has been observed in neural and epithelial tissues, enabling studies on morphogen regulation, ligand trafficking, and receptor-mediated endocytosis[1][2]. Agonists and inhibitors targeting LRP2 remain under investigation, with potential applications for modulating SHH pathways in developmental disorders and tissue repair[2]. Collectively, LRP2 functions at the interface of morphogen signaling and cellular uptake, offering a distinct mechanistic profile within the LDLR receptor family and a valuable target for experimental manipulation[1][2].
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Subcellular Localization
Apical cell membrane; Single-pass type I membrane protein; Endosome lumen; Membrane, coated pit; Cell projection, dendrite; Cell projection, axon
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Expression
Tissue_specificity:In the inner ear, it is highly expressed in the marginal cells of the stria vascularis (protein level) (PubMed:17846082) . In the female reproductive tract, it is expressed in the luminal side of the uterine epithelium (protein level) (PubMed:16143106) . In the adult brain, it is expressed in the ependymal cells of the lateral ventricles, and expression is limited to the ependyma facing the stem cell microenvironment (protein level) (PubMed:20460439) . It is expressed in neurons throughout the brain, including the hippocampus, limbic cortex, and cerebellum (protein level) (PubMed:20637285) . In the developing optic nerve, it is expressed only in astrocytes at 14.5 dpc, 16.5 dpc, and 18.5 dpc (protein level) (PubMed:22354480) .
Induction:Down-regulated in the kidney by cannabinoids, such as endocannabinoid anandamide and synthetic cannabinoid HU-210 -
Subunit
Binds plasminogen, extracellular matrix components, plasminogen activator-plasminogen activator inhibitor type I complex, apolipoprotein E-enriched beta-VLDL, lipoprotein lipase, lactoferrin, CLU/clusterin and calcium. Forms a multimeric complex together with LRPAP1 (By similarity). Interacts (via PxLPxI/L motif) with ANKRA2 (via ankyrin repeats) (By similarity). Interacts with LRP2BP. Interacts (via NPXY motif) with DAB2; the interaction is not affected by tyrosine phosphorylation of the NPXY motif (PubMed:11247302). Interacts with MB (By similarity). Interacts with BMP4 (PubMed:15623804). Interacts with the Sonic hedgehog protein N-product which is the active product of SHH (PubMed:11964399). Interacts with CST3 in a calcium-dependent manner (By similarity). Interacts with the vitamin-D binding protein GC/DBP (PubMed:10052453). Interacts with sex hormone-binding protein SHBG (By similarity). Interacts with angiotensin-2 (PubMed:15467006). Also interacts with angiotensin 1-7 (PubMed:16380466). Interacts with APOM (PubMed:16099815). Interacts with selenoprotein SEPP1 (PubMed:18174160). Interacts with LEP (By similarity). Interacts with ALB (By similarity). Interacts with the antiapoptotic protein BIRC5/survivin (By similarity). Interacts with matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1 (By similarity). In neurons, forms a trimeric complex with APP and APPB1/FE65 (PubMed:20637285). Interacts with LDLRAP1/ARH; mediates trafficking of LRP2 to the endocytic recycling compartment (By similarity). Does not interact with beta-amyloid protein 40 alone but interacts with the complex composed of beta-amyloid protein 40 and CLU/APOJ (By similarity). Interacts with MDK (PubMed:10772929)
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SwissProt ID
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Synonyms
LRP2_HUMAN; Low-density lipoprotein receptor-related protein 2; LDL receptor related protein 2; DBS; GP330; LRP-2; Glycoprotein 330(gp330); Megalin;
Documentation
References
[1]. Spuch C, et al. LRP-1 and LRP-2 receptors function in the membrane neuron. Trafficking mechanisms and proteolytic processing in Alzheimer's disease. Front Physiol. 2012 Jul 16;3:269. [Content Brief]
[2]. Christ A, et al. LRP2, an auxiliary receptor that controls sonic hedgehog signaling in development and disease. Dev Dyn. 2016 May;245(5):569-79. [Content Brief]
[3]. Marzolo MP, et al. New insights into the roles of megalin/LRP2 and the regulation of its functional expression. Biol Res. 2011;44(1):89-105. [Content Brief]
[4]. Zilberberg A, et al. The low density lipoprotein receptor-1, LRP1, interacts with the human frizzled-1 (HFz1) and down-regulates the canonical Wnt signaling pathway. J Biol Chem. 2004 Apr 23;279(17):17535-42. [Content Brief]
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