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BRN3A Antibody (YA6486)

(Synonyms: Brain specific homeobox/POU domain protein 3A antibody; Brain-3A antibody; Brain-specific homeobox/POU domain protein 3A antibody; Brn 3.0 antibody; BRN 3A antibody; Brn-3A antibody; Brn3 antibody; BRN3A antibody; class 4 antibody; FLJ13449 antibody; Brain specific homeobox/POU domain protein 3A antibody; Brain-3A antibody; Brain-specific homeobox/POU domain protein 3A antibody; Brn 3.0 antibody; BRN 3A antibody; Brn-3A antibody; Brn3 antibody; BRN3A antibody; class 4 antibody; FLJ13449 antibody; Homeobox/POU domain protein RDC 1 antibody; Homeobox/POU domain protein RDC-1 antibody; Homeobox/POU domain protein RDC1 antibody; Oct T1 antibody; Oct-T1 antibody; OctT1 antibody; PO4F1_HUMAN antibody; POU class 4 homeobox 1 antibody; POU domain antibody; POU Domain Class 4 Transcription Factor 1 antibody; Pou4f1 antibody; RDC 1 antibody; RDC1 antibody; transcription factor 1 antibody; )
Customer Review

Based on 1 Customer Validation

BRN3A Antibody (YA6486) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRN3A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-F, IHC-P, WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BRN3A Antibody (YA6486)
  • Experimental Validation Results for BRN3A Antibody (YA6486)
  • BRN3A Antibody (YA6486) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
Dilution Ratio 1:500 1:50-1:200 1:1000

Product Details

Description

BRN3A Antibody (YA6486) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRN3A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719213

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BRN3A Antibody (YA6486)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BRN3A Antibody (YA6486)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    BRN3A multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Activates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site |Able to act as transcription factor, cannot regulate the expression of the same subset of genes than isoform 1. Does not have antiapoptotic effect on neuronal cells[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Nucleus,Cytoplasm

  • Expression


    Tissue_Specificity: Expressed in mature osteoclasts (at protein level) (PubMed:17668438). Brain, peripheral sensory nervous system and retina (PubMed:8162704). In the adult nervous system, predominates in the medial habenula, superficial gray of the superior colliculus, red nucleus, mesencephalic nucleus of the trigeminal ganglion, nucleus ambiguus, inferior olivary nucleus, and peripheral sensory ganglia (PubMed:8290353)
    Induction: Up-regulated by the osteoclast differentiation factor TNFSF11 (PubMed:17668438)

  • Isoforms & Post-Translational Modification

    P17208 has two isomers: P17208-1: 42767 Da (predicted); P17208-2: 33561 Da (predicted).

  • Subunit

    Interacts (via N-terminus) with RIT2; the interaction controls POU4F1 transactivation activity on some neuronal target genes (PubMed:12934100)

  • SwissProt ID

    P17208

  • Gene ID
    18996 [NCBI]
  • Synonyms

    Brain specific homeobox/POU domain protein 3A antibody; Brain-3A antibody; Brain-specific homeobox/POU domain protein 3A antibody; Brn 3.0 antibody; BRN 3A antibody; Brn-3A antibody; Brn3 antibody; BRN3A antibody; class 4 antibody; FLJ13449 antibody; Brain specific homeobox/POU domain protein 3A antibody; Brain-3A antibody; Brain-specific homeobox/POU domain protein 3A antibody; Brn 3.0 antibody; BRN 3A antibody; Brn-3A antibody; Brn3 antibody; BRN3A antibody; class 4 antibody; FLJ13449 antibody; Homeobox/POU domain protein RDC 1 antibody; Homeobox/POU domain protein RDC-1 antibody; Homeobox/POU domain protein RDC1 antibody; Oct T1 antibody; Oct-T1 antibody; OctT1 antibody; PO4F1_HUMAN antibody; POU class 4 homeobox 1 antibody; POU domain antibody; POU Domain Class 4 Transcription Factor 1 antibody; Pou4f1 antibody; RDC 1 antibody; RDC1 antibody; transcription factor 1 antibody;

BRN3A Antibody (YA6486) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • BRN3A Antibody (YA6486) Product Image
    1/16
  • Experimental Validation Results for BRN3A Antibody (YA6486)
  • Experimental Validation Results for BRN3A Antibody (YA6486)
  • BRN3A Antibody (YA6486) Product Image
  • Experimental Validation Results for BRN3A Antibody (YA6486)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BRN3A Antibody (YA6486)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BRN3A Antibody (YA6486)
  • Experimental Validation Results for BRN3A Antibody (YA6486)
  • BRN3A Antibody (YA6486) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • BRN3A Antibody (YA6486) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using BRN3A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • BRN3A Antibody (YA6486) Product Image IHC-P
  • BRN3A Antibody (YA6486) Product Image IHC-P