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Caspase-8 (p18) Antibody (YA6528)

(Synonyms: ALPS2B antibody; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody; Apoptosis related cysteine peptidase antibody; Apoptotic cysteine protease antibody; Apoptotic protease Mch-5 antibody; Apoptotic protease Mch5 antibody; CAP 4 antibody; CAP4 antibody; CASP-8 antibody; CASP8 antibody; ALPS2B antibody; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody; Apoptosis related cysteine peptidase antibody)
Customer Review

Based on 1 Customer Validation

Caspase-8 (p18) Antibody (YA6528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-8 (p18).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Caspase-8 (p18) Antibody (YA6528) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:2000 1:100

Product Details

Description

Caspase-8 (p18) Antibody (YA6528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-8 (p18).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 43/18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

Synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719222

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
    Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg), NIH/3T3 (lane3, 20μg), and RAW264.7 (lane4, 20μg) using Caspase-8 (p18) Antibody (HY-P86835). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
    Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
    Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Caspase 8 is an initiator protease of death receptor-mediated extrinsic apoptosis and also supports cell survival by restraining RIP-family kinase-dependent necroptosis[1]. Mechanistically, caspase 8 works with cFLIP to regulate the switch among survival, apoptosis, and necroptosis, while cFLIP is structurally related to caspase 8 but lacks proteolytic activity[1][2]. Therefore, caspase 8 differs from inflammatory caspases such as caspase 1, 4, 5, and 12, which participate in inflammasome-related innate immune responses[3]. In intestinal disease, caspase 8 controls Paneth cell necroptosis and may contribute to epithelial cell death and mucosal inflammation in Crohn’s disease[3]. In severe SARS-CoV-2 mouse models, non-apoptotic caspase 8 promoted cytokine release, IL-1β elevation, NF-κB-linked inflammation, and disease pathology[4]. In ovarian cancer, low caspase 8 expression correlated with shorter overall survival, while caspase 8 loss enhanced non-apoptotic necroptotic death under NF-κB blockade[5]. For experimental applications, studies use genetic caspase 8 loss, viral caspase 8 inhibitors, and necroptosis pathway modulation to separate apoptosis, inflammation, and RIPK3-MLKL-dependent cell death[6].

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Expression


    Tissue_Specificity: Expressed in a wide variety of tissues. Highest expression in spleen, thymus, lung, liver and kidney. Lower expression in heart, brain, testis and skeletal muscle

  • Isoforms & Post-Translational Modification

    O89110: 480 amino acids, molecular weight 55357 Da.
    MARylated by PARP12; MARylation is required for basolateral export of E-Cadherin

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit (By similarity)

  • SwissProt ID

    O89110

  • Gene ID
    12370 [NCBI]
  • Synonyms

    ALPS2B antibody; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody; Apoptosis related cysteine peptidase antibody; Apoptotic cysteine protease antibody; Apoptotic protease Mch-5 antibody; Apoptotic protease Mch5 antibody; CAP 4 antibody; CAP4 antibody; CASP-8 antibody; CASP8 antibody; ALPS2B antibody; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody; Apoptosis related cysteine peptidase antibody

References

[1]. Salvesen GS, et al. Functions of caspase 8: the identified and the mysterious. Semin Immunol. 2014 Jun;26(3):246-52. [Content Brief]

[2]. Tsuchiya Y, et al. FLIP the Switch: Regulation of Apoptosis and Necroptosis by cFLIP. Int J Mol Sci. 2015 Dec 18;16(12):30321-41. [Content Brief]

[3]. Becker C, et al. Complex roles of caspases in the pathogenesis of inflammatory bowel disease. Gastroenterology. 2013 Feb;144(2):283-293. [Content Brief]

[4]. M Bader S, et al. Non-apoptotic caspase-8 is critical for orchestrating exaggerated inflammation during severe SARS-CoV-2 infection. Nat Commun. 2025 Nov 13;16(1):9822. [Content Brief]

[5]. Kim M, et al. Caspase 8 expression may determine the survival of women with ovarian cancer. Cell Death Dis. 2016 Jan 14;7(1):e2045. [Content Brief]

[6]. Daley-Bauer LP, et al. Mouse cytomegalovirus M36 and M45 death suppressors cooperate to prevent inflammation resulting from antiviral programmed cell death pathways. Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):E2786-E2795. [Content Brief]

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Caspase-8 (p18) Antibody (YA6528) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg), NIH/3T3 (lane3, 20μg), and RAW264.7 (lane4, 20μg) using Caspase-8 (p18) Antibody (HY-P86835). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Caspase-8 (p18) Antibody (YA6528) Product Image
    1/16
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Caspase-8 (p18) Antibody (YA6528) Product Image
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg), NIH/3T3 (lane3, 20μg), and RAW264.7 (lane4, 20μg) using Caspase-8 (p18) Antibody (HY-P86835). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Experimental Validation Results for Caspase-8 (p18) Antibody (YA6528)
  • Caspase-8 (p18) Antibody (YA6528) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg), NIH/3T3 (lane3, 20μg), and RAW264.7 (lane4, 20μg) using Caspase-8 (p18) Antibody (HY-P86835). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Caspase-8 (p18) Antibody (YA6528) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Caspase-8 (p18) Antibody (YA6528) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Caspase-8 (p18) With Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Caspase-8 (p18) antibody (HY-P86835) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Caspase-8 (p18) Antibody (YA6528) Product Image WB
  • Caspase-8 (p18) Antibody (YA6528) Product Image ICC
  • Caspase-8 (p18) Antibody (YA6528) Product Image ICC