Mannose Binding Protein Antibody
(Synonyms: Mannose-binding protein C, MBP-C, Mannan-binding protein, RA-reactive factor P28A subunit, RARF/P28A, Mbl2)Mannose Binding Protein Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Mannose Binding Protein.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
Mannose Binding Protein Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Mannose Binding Protein.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 27 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26 kDa
Synthetic peptide corresponding to the center region of human Mannose Binding Protein.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Mannose Binding Protein is a Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Specifically recognizes and binds the mannose moiety of carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response. May bind DNA[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Secreted; Cell surface
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Expression
Tissue_Specificity: Plasma protein produced mainly in the liver. -
Isoforms & Post-Translational Modification
Mannose Binding Protein has an amino acid length of 248, molecular weight is 26144 Da.
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Subunit
Oligomeric complex of 3 or more homotrimers.
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SwissProt ID
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Synonyms
Mannose-binding protein C, MBP-C, Mannan-binding protein, RA-reactive factor P28A subunit, RARF/P28A, Mbl2
Documentation
[1]. Nauta AJ, et al. Mannose-binding lectin engagement with late apoptotic and necrotic cells. Eur J Immunol. 2003 Oct;33(10):2853-63. [Content Brief]
[2]. Degn SE, et al. Mannan-binding lectin-associated serine protease (MASP)-1 is crucial for lectin pathway activation in human serum, whereas neither MASP-1 nor MASP-3 is required for alternative pathway function. J Immunol. 2012 Oct 15;189(8):3957-69. [Content Brief]
[3]. Sheriff S, et al. Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil. Nat Struct Biol. 1994 Nov;1(11):789-94. [Content Brief]
[4]. Thiel S, et al. A second serine protease associated with mannan-binding lectin that activates complement. Nature. 1997 Apr 3;386(6624):506-10. [Content Brief]
[5]. Super M, et al. Association of low levels of mannan-binding protein with a common defect of opsonisation. Lancet. 1989 Nov 25;2(8674):1236-9. [Content Brief]
[6]. Kawasaki N, et al. Isolation and characterization of a mannan-binding protein from human serum. J Biochem. 1983 Sep;94(3):937-47. [Content Brief]
[7]. van Emmerik LC, et al. Binding of mannan-binding protein to various bacterial pathogens of meningitis. Clin Exp Immunol. 1994 Sep;97(3):411-6. [Content Brief]
[8]. Stravalaci M, et al. Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. Nat Immunol. 2022 Feb;23(2):275-286. [Content Brief]
[9]. Palaniyar N, et al. Nucleic acid is a novel ligand for innate, immune pattern recognition collectins surfactant proteins A and D and mannose-binding lectin. J Biol Chem. 2004 Jul 30;279(31):32728-36. [Content Brief]
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