MAP3K4 Antibody (YA3050)(PBS only)

Cat. No.: HY-P83305A: Data Sheet User Guide for Antibodies
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MAP3K4 Antibody (YA3050) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAP3K4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

MAP3K4 Antibody (YA3050) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAP3K4.

Host

Rabbit

Molecular Weight

Predicted band size: 182 kDa;

Observed band size: 200 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y6R4

Gene ID

4216 [NCBI]

Immunogen

A synthetic peptide of human MAP3K4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6

Subcellular Localization：Cytoplasm, perinuclear region

Expression：
Tissue_specificity:Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues

Isoforms & Post-Translational Modification：Q9Y6R4 has 2 isomers: Q9Y6R4-1: 181685 Da (predicted); Q9Y6R4-2: 177014 Da (predicted).

Subunit：Monomer and homodimer. Homodimerization enhances kinase activity. Interacts with TRAF4; this promotes homodimerization (PubMed:16157600). Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with AXIN1 and DIXDC1; interaction with DIXDC1 prevents interaction with AXIN1 (PubMed:15262978). Interacts with GADD45 and MAP2K6 (PubMed:12052864). Interacts with ZFP36; this interaction enhances the association with SH3KBP1/CIN85 (PubMed:20221403). Interacts with SH3KBP1; this interaction enhances the association with ZFP36 (PubMed:20221403). Interacts with CDC42 (By similarity)

Research Field

Signal Transduction

Synonyms

MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sk-br-3-cell.html [Content Brief]