CCR9 Antibody (YA3119)

Cat. No.: HY-P83374: Data Sheet COA
SDS
User Guide for Antibodies
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CCR9 Antibody (YA3119) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCR9.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CCR9 Antibody (YA3119) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CCR9 Antibody (YA3119) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCR9.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P51686

Gene ID

10803 [NCBI]

Immunogen

A synthesized peptide derived from human CCR9 aa1-48.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from HUVEC(lane 2(20ug) , A375(lane 3(20ug) and HepG2(lane 4(20ug) , Raji(lane 5(20ug) using CCR9 Antibody(HY-P83374) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Daudi (lane 3, 15 μg), U937 (lane 4, 15 μg), Hela (lane 5, 15 μg), and HT-29 (lane 6, 15 μg) using CCR9 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Immunocytochemistry analysis of HeLa cells labeling CCR9 with CCR9 Antibody (HY-P83374) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CCR9 Antibody (HY-P83374) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling CCR9 with CCR9 Antibody (HY-P83374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CCR9 Antibody (HY-P83374) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level; (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Highly expressed in the thymus and low in lymph nodes and spleen

Isoforms & Post-Translational Modification：P51686 has 2 isomers: P51686-1: 42016 Da (predicted); P51686-2: 40713 Da (predicted).

Database

Entrez Gene: 10803 Human ; 12769 Mouse ; 282832 Rat

SwissProt: P51686 Human ; Q9WUT7 Mouse ;

OMIM: 604738 Human

Research Field

Immunology

Synonyms

G-protein coupled receptor 28; GPR-9-6; CDw199; CCR9

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CCR9 Antibody (YA3119) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CCR9 Antibody (YA3119)

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Background

Documentation

CCR9 Antibody (YA3119) Related Classifications

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